HCV-RNA与HCV-cAg检测的相关性分析  

Study on correlation analysis on serum HCV - RNA and HCV - cAg

作　　者：苏明权[1] 杨柳[1] 王娟[1] 常亮[1] 肖凤静[1] 马越云[1] 郝晓柯[1] 

机构地区：[1]第四军医大学西京医院检验科,陕西西安710032

出　　处：《临床和实验医学杂志》2013年第17期1362-1363,共2页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨丙型肝炎病毒核心抗原(HCV-cAg)与HCV-RNA检测的相关性及其在丙型肝炎病毒感染诊断中的价值。方法采用双抗体夹心酶联免疫分析法(ELISA)检测HCV-cAg;采用实时荧光定量PCR法检测HCV-RNA,以了解两者检测方法的相关性。结果在160例HCV-RNA阳性血清中,HCV-cAg阳性148例,阳性符合率92.5%;在60例HCV-cAg阳性血清中,HCV-RNA阳性59例,阳性符合率98.3%。结论通过对HCV-RNA与HCV-cAg检测,说明HCV核心抗原与HCV-RNA检测可作为反映HCV复制的间接指标,预防窗口期感染。由于HCV-cAg在方法学上与HCV-RNA相比,具有方法简便、快速、价廉,所需设备简单,易于普及应用等优点,特别是在不具备HCV-RNA检测条件的基层医疗单位作为HCV感染检测的直接证据具有重要的意义。Objective To explore the diagnostic significance of serum hepatitis C virus core antigen （ HCV - cAg） and HCV - RNA during hepatitis C virus infection. Methods The rate of diagnostic coincidence was evaluated after the detection of serum HCV - cAg and HCV - RNA by ELISA and real - time PCR. Results A total of 148 HCV - cAg positive results were found out from 160 HCV - RNA positive serum samples, while 59 HCV - RNA positive results were found out from 60 HCV - cAg positive samples, and the rates of positive coincidence were 92. 5% and 98.3%, respectively. Conclusion The detection of HCV - tag and HCV - RNA would likely be served as indirect diagnostic markem for clinical application in prevention of window period infeetion. The detection of HCV - cAg by ELISA is easily applied for the reasons of its simple, rapid and cheap, especially in community hospital since it provides the evidence of HCV infection.

关 键 词：丙型肝炎病毒（HCV） 核心抗原 HCV-RNA 

分 类 号：R446.6[医药卫生—诊断学]