氧自由基清除剂对急性肺损伤大鼠核因子-κB的作用及机制研究  

The effect and mechanism study of oxygen free radical scavenger on nuclear factor-κB in rat acute lung injury

作　　者：王婷[1] 常天才[1] 岳卫平[1] 

机构地区：[1]长治医学院附属和济医院呼吸内科,山西长治046011

出　　处：《中国当代医药》2013年第26期10-11,14,共3页China Modern Medicine

摘　　要：目的探讨氧自由基清除剂依达拉奉对脂多糖诱导的急性肺损伤大鼠核因子-κB(NF-κB)的作用及机制。方法将30只雄性SD大鼠随机分为3组(n=10):对照组(A组)、模型组(B组)、依达拉奉组(C组)。B、C组大鼠腹腔注射脂多糖5 mg/kg进行造模,A组大鼠腹腔注射0.9%氯化钠注射液5 mg/kg进行对照。造模后C组大鼠腹腔注射依达拉奉10 ml/kg进行干预,A、B组大鼠腹腔注射0.9%氯化钠注射液10 ml/kg进行对照。6 h后处死大鼠,抽取腹主动脉血液行血气分析测定血氧分压(PO2),用免疫组织化学法测定肺组织中NF-κB的含量,用ELISA测定TNF-α的含量。结果 B、C组大鼠PO2低于A组,差异有统计学意义(P<0.05);C组大鼠PO2的下降幅度小于B组,与B组相比,C组的PO2高,差异有统计学意义(P<0.05)。B、C组大鼠NF-κB、TNF-α高于A组,差异有统计学意义(P<0.05);C组大鼠NF-κB、TNF-α的上升幅度小于B组,与B组相比,C组的NF-κB、TNF-α低,差异有统计学意义(P<0.05)。结论依达拉奉对脂多糖致急性肺损伤大鼠肺组织起保护作用,其机制与抑制肺组织NF-κB的表达从而抑制肺部炎症反应有关。Objective To explore the effect and mechanism of oxygen free radical scavenger edaravone on nuclear fac- tor-KB of in rat acute lung injury induced by lipopolysaccharide. Methods 30 male SD rats were evenly and randomly divided into three groups：control group （group A）,model group （group B）,and edaravone group （group C）.In group B and group C,5 mg/kg lipopolysaccharide was injected in peritoneal cavity respectively for modeling.In group A,5 mg/kg of 0.9% sodium chloride injection was injected in abdominal cavity as control group.After modeling,in group C,intraperi- toneal injection of 10 ml/kg edaravone was performed for intervention.The intraperitoneal injection of 10 ml/kg in 0.9% sodium chloride injection was carried out in both group A and group B for comparisons.These rats were killed after 6 hours.The blood oxygen pressure （POe） was measured by blood gas analysis after blood being extracted from abdominal aorta,the content of nuclear factor-KB in lung tissue was determined by immunohistochemistry,and the content of TNF-oL was tested by ELISA. Results The PO2 of rats in both group B and C were lower than that in group A with statistical difference （P〈O.05）.The descending range of PO2 in group C was less than that in group B.In comparison with group B,the PO2 in group C was higher with statistical difference （P〈O.05）.The contents of nuclear factor-KB and TNF-cL of rats in group B and C were higher than those in group A with statistical differences （P〈0.05）.The ascending range of nuclear fact0r-KB and TNF-ot in group C was less than those in group B.Compared with group B,the contents of nuclear factor-KB and TNF-a in group C were lower, which displayed statistical differences （P〈0.05）. Conclusion Edaravone has the protective effect on lung tissue of rat in acute lung injury caused by lipopolysaccharide,and is relat- ed with inhibition of NF-KB expression in lung tissue and inhibition of pulmonary inflammatory response aeeordingly.

关 键 词：自由基清除剂 急性肺损伤 核因子-ΚB 

分 类 号：R563[医药卫生—呼吸系统]