维多利亚蓝B-分光光度法测定利福平  

Spectrophotometric Determination of Rifampicin by the Color Reaction of Victoria Blue B

作　　者：江虹[1] 周晋[1] 刘艳[1] 庞向东[1] 方卢秋[1] 

机构地区：[1]长江师范学院化学化工学院,重庆408100

出　　处：《理化检验（化学分册）》2013年第8期940-942,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：重庆市教委科技基金资助项目(KJ121320)

摘　　要：在pH6．92的Tris-盐酸缓冲溶液中，维多利亚蓝B与利福平生成络合物，最大吸收波长为562nm。利福平的浓度在8．0×10-6。tool·L-1范围内符合比耳定律，表观摩尔吸光率为1．86×10……5L·mol-1·cm-1。方法用于利福平胶囊中利福平含量的测定，测定值与标示量相一致。加标回收率在98．2％～100%之间，相对标准偏差（n=6）均小于1.5％。In a Tris-HCl buffer solution of pH 6.92, a complex was formed by the reaction of rifampicin with victoria blue B, having its absorption maximum at the wavelength of 562 nm. Beer＇s law was kept in the range within 8. 0 X 10 -6 mol L-1 , with apparent molar absorptivity of 1.86 ;〈 10. tool-1 . cni The proposed method was used in the determination of rifampicin in its capsules, and the results obtained were in conformity with the labeled values. Values of recovery found by standard addition method were in the range of 98. 2--100 with RSD＇s （n=6） less than 1.5.

关 键 词：维多利亚蓝B 利福平 显色反应 分光光度法 

分 类 号：O657.3[理学—分析化学]