ERK1／2和蝴路介导肾素前体诱导的大鼠血管平滑肌细胞增殖  

Prorenin Induces Rat Vascular Smooth Muscle Cell Proliferation via ERK1/2 and Akt Activation Pathway

作　　者：曹学照[1] 艾春雨[1] 刘钢[1] 马虹[1] 

机构地区：[1]中国医科大学附属第一医院麻醉科,辽宁沈阳110001

出　　处：《中国血液流变学杂志》2013年第2期226-228,共3页Chinese Journal of Hemorheology

摘　　要：目的探讨肾素前体（prorenin）诱导大鼠血管平滑肌细胞的增殖是否通过ERKl／2和Akt通路。方法利用[3H]同位素标记的胸苷的结合检测血管平滑肌细胞增殖。蛋白印记分析检测肾素前体诱导的平滑肌细胞ERK和Akt的活化。结果肾素前体剂量依赖性诱导ERK和Akt磷酸化，在20min达到高峰。20nmol／L肾素前体处理细胞48h后细胞内[3H]同位素标记的胸苷含量增多（P〈0．05），这种促进细胞增殖的作用可以被预处理ERK激酶抑制剂PD98059（100μmol／L）SFIPl3K激酶抑制剂wortmannin（100nmol／L）阻断。结论实验结果表明。肾素前体诱导血管平滑肌细胞产生的增殖是通过ERKl／2和Akt通路发挥作用。Objective To investigate the effect of prorenin on vascular smooth muscle cell（VSMC） proliferation via ERK and Akt activation dependent pathway.Methods DNA synthesis in rat VSMCs was measuring by [3H] thymidine.Extracellular signal-regulated kinase（ERK） and Akt phosphorylation were measured by Western blotting.Results Rat recombinant prorenin dose-dependently induced ERK and Akt phosphorylation with a peak at 20min. Prorenin（20nmol/L,48h） significantly increase VSMC [3HI thymidine incorporation,which attenuated by pretreatment with ERK kinasc inhibitor, PD98059（100 μmol/L）,and PI3 kinase inhibitors,wortmannin（100nmol/L）.Conclusion ERK and Akt activation contributes to prorenin-induced oroliferative effects in VSMCs.

关 键 词：肾素前体 ERKL 2 Akt 大鼠血管平滑肌细胞 

分 类 号：R614.1[医药卫生—麻醉学]