18株南海海洋真菌的初步鉴定及其发酵产物的细胞毒活性和抗菌活性筛选  

Preliminary identification of 18 marine fungal strains from the South China Sea and screening of their fermentation products for cytotoxic and antibacterial activities

作　　者：张玲[1,2,3] 张庆波[1,3] 陈玉婵[2] 李浩华[2] 王磊[2] 章卫民[2] 

机构地区：[1]中国科学院南海海洋研究所,广东广州510301 [2]广东省微生物研究所,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,省部共建华南应用微生物国家重点实验室,广东广州510070 [3]中国科学院大学,北京100049

出　　处：《热带海洋学报》2013年第3期47-51,共5页Journal of Tropical Oceanography

基　　金：国家自然科学基金项目(31272087);广东省科技计划项目(2011B031200003);中国科学院知识创新工程重要方向项目(KSCX2-EW-G-12)

摘　　要：从南海海洋沉积物中分离得到18株真菌,经内转录间隔区(ITS)序列测定进行初步鉴定;采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法对其发酵液及菌丝体的提取物进行体外细胞毒活性和抗菌活性筛选。结果显示,对人乳腺癌细胞株MCF-7增殖的抑制率在90%以上的菌株有5株;对金黄色葡萄球菌的抑制率在90%以上的有15株,占分离菌株总数的83.3%;对大肠杆菌和铜绿假单胞菌的抑制率在90%以上的各有2株,其中有1株对以上3种供试细菌都表现出较强的抑制活性,抑制率达90%以上。Eighteen strains of marine fungi were isolated from the sediments of the South China Sea and primarily identified by intergenic transcribed spacer （ITS） sequencing. Cytotoxic and antibacterial activities of their fermentation products were evaluated using the 3-（4,5）-dimethylthiahiazo（-z-y1）-3,5-di-phenytetrazoliumromide （MTT） method. The results showed that five strains displayed cytotoxic activity against human breast adenocarcinoma cell line （MCF-7） with an inhibition rate of above 90%; fifteen strains exhibited bacterial inhibition against Staphylococcus aureusat the rate above 90%, accounting for 83.3% of the total strains isolated; two strains showed bacterial inhibition against Escherichia coliat the rate above 90% and two strains showed bacterial inhibition against Pseudomonas aeruginosaat the rate above 90%; and one strain was found to show strong bacterial inhibition against the three tested bacteria.

关 键 词：海洋真菌 细胞毒 抗菌 

分 类 号：P735.51[天文地球—海洋生物学]