BMSCs分化为NCs的拉曼光谱研究  

The Study on Raman Spectrum of Bone Marrow Mesenchymal Stem Cells into Neural Cells

作　　者：张晓利[1] 柳璐[2] 刁振琦[1] 李云涛[1] 熊洋[1] 张建民[1] 唐伟跃[1] 

机构地区：[1]郑州大学物理工程学院,河南郑州450001 [2]郑州铁路职业技术学院护理学院,河南郑州450052

出　　处：《郑州大学学报（理学版）》2013年第3期77-80,97,共5页Journal of Zhengzhou University:Natural Science Edition

摘　　要：分离培养大鼠BMSCs并诱导分化为NCs,应用拉曼光谱仪测定两种细胞的光谱,分析细胞内部蛋白质、核酸、脂类等含量、构象和构型变化.结果发现,骨髓间充质干细胞的拉曼光谱与神经细胞之间存在较大的差异.表现为:646 cm-1处和719 cm-1处的拉曼峰在神经细胞内消失;神经细胞在1 603 cm-1处出现新的拉曼峰,归属为苯丙氨酸和酪氨酸的C—C弯曲振动;1 738 cm-1处拉曼峰在神经元样细胞中频移到1 746 cm-1处.试验表明拉曼光谱有望成为鉴别BMSCs与NCs的一种有效新方法.Isolated and cultured rat bone marrow mesenchymal stem cells（BMSCs） were induced to dif- ferentiate into neural cells （NCs）. The BMSCs and NCs were analyzed by the change of configuration, the relative content of nucleic acid, lipid and protein using laser Raman spectrometer. Significant differ- ence in the average spectra of BMSCs and NCs was found： the peaks at 646 cm-1 and 719 cm-1 disap- showed that Raman spectrum was expected to become a new method to identificate BMSCs and NCs.

关 键 词：骨髓间充质干细胞 神经细胞 拉曼光谱 分化 

分 类 号：O657.3[理学—分析化学]