“禽康散”对人工感染NDV肉鸡红细胞免疫功能的影响  

The changes of red blood cell immune function by complex Chinese crude drug“Qinkangsan”on chicken artificially challenged by NDV

作　　者：沈留红[1] 邓惠丹[1] 邓俊良[1] 杨军[1,2] 申渝波[1,3] 左之才[1] 王娅[1] 任志华[1] 

机构地区：[1]四川农业大学动物医学院/动物疫病与人类健康四川省重点实验室/动物环境公害性疾病四川省重点实验室,四川雅安625014 [2]毕节市农牧局,贵州毕节551700 [3]通威集团药物研究所,四川成都620000

出　　处：《扬州大学学报（农业与生命科学版）》2013年第2期16-19,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：长江学者和创新团队发展计划项目(IRT0848);四川省应用基础研究计划项目(2008JY0098);四川省教育厅自然科学重点项目(2006A009);四川农业大学优秀学士论文培育计划项目(00209050)

摘　　要：将250只21日龄健康艾维茵肉鸡随机均分为5组,第1～3组(治疗组)用新城疫病毒(NDV)强毒株攻毒,并在22～25日龄肉鸡饲料中分别添加0.5%、1.0%、2.0%的中药"禽康散",第4组(阴性对照组)、第5组(阳性对照组)不用药,探讨"禽康散"对人工感染NDV肉鸡红细胞免疫功能的影响。结果表明:添加0.5%、1.0%中药组红细胞免疫复合花环率在攻毒3d后(用药2d后)均明显低于阳性对照组(P<0.01);红细胞补体受体花环率高于阳性和阴性对照组,且在攻毒后3d(用药后2d)、7d(停药后2d)时差异达到极显著(P<0.01)。证明"禽康散"具有促进肉鸡红细胞免疫功能,提高机体抗NDV的能力,且添加浓度为1.0%时效果最佳。In order to investigate the influence of complex Chinese crude drug ＂Qinkangsan＂ on red blood cell immune functions of broiler chickens artificially infected with NDV, 250 21-day-old broiler chickens were randomly divided into 5 groups. Group 1- 3 were challenged with virulent NDV-F48E9, then fed with 0.5 %, 1.0 %, 2.0 % ＂Qinkangsan＂ for 4 days, respectively. Group 4 and 5 were treated as negative and positive control respectively. The results showed that the ICR rosette percentage of the groups fed with 0.5 %and 1 % ＂Qinkangsan＂ were significantly lower than that of positive control group （P〈0.01） at 3 d post-challenge （2 days after drug feed） ; and the erythrocyte C3bR rosette percentage of the two treated groups were higher than that of positive and negative control groups , especially significant at 3 d （2 days after drug feed）, 7 d （2 days after drug termination） post-challenge （P〈0.01）. Therefore,the complex Chinese crude drug ＂Qinkangsan＂ could improve red blood cell immune functions and enhance resistance to NDV of broiler chick- ens, especially at the concentration of 1.0 %.

关 键 词：“禽康散” 新城疫病毒 红细胞免疫 

分 类 号：S858.31[农业科学—临床兽医学]