玉米株高性状QTL的贝叶斯定位分析  

Bayesian mapping of quantitative trait loci controlling plant height in maize(Zea mays L.)

作　　者：胡文明[1] 汤在祥[1,2] 张恩盈[1] 徐辰武[1] 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室,江苏扬州225009 [2]苏州大学医学部公共卫生学院,江苏苏州215123

出　　处：《扬州大学学报（农业与生命科学版）》2013年第2期41-46,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家重点基础研究发展计划项目(973-2011CB100106);国家自然科学基金资助项目(30971846;31171187;31100882);江苏省高等学校自然科学研究重大项目(09KJA210002)

摘　　要：为探索控制玉米株高的主效QTL、互作QTL及其遗传结构,通过基于贝叶斯理论的R/qtlbim QTL定位分析软件包,对一个玉米F2:3群体株高性状进行QTL分析。通过一维扫描,初步检测到8个主效QTL,分别位于第3～7染色体上,单个QTL的贝叶斯因子(以2logBF衡量)为2.237～6.196。通过二维后验概率扫描发现大量较弱的互作信号,多数集中在3～7染色体上。通过贝叶斯因子分析,获得控制玉米株高的最优遗传结构,该遗传结构包含6个主效应QTL,QTL间不存在互作效应。逐步回归拟合分析表明,该遗传结构达到极显著水平,可解释37.383%表型变异,6个QTL均达到极显著水平,单个QTL的表型变异贡献为3.924%～10.776%。这表明,株高的遗传结构相对较为简单,QTL互作对株高的影响较小,可忽略不计。In order to explore the main effect, interactive effect and genetic architecture of plant height in maize, an F2,3population was used to map QTLs controlling plant height of maize by QTL mapping software package R/qtlbim based on Bayesian statistics. With the one-dimension scan, a total of eight QTLs were mapped on chromosome 3 to 7, and the 21ogBF of each QTL ranged from 2. 237 to 6. 196. Two-dimension scan showed that there existed a large number of interactive signals with weaker effects. These interaction signals were also concentrated on chromosome 3 to 7. With the Bayes factor analysis, an optimal genetic architecture of plant height in this population was obtained, and it was composed of six main effects QTLs without interaction. The stepwise regression analysis further indicated that the genetic architec- ture was significant at 0. 001 levels and could explain 37.33% of phenotype variation. Each of six QTLs was very signifi- cant and could explain 3. 924% to 10. 776 % of phenotype variation. This result suggested that the genetic architecture for plant height was relative simple and the interaction among QTLs might be ignored.

关 键 词：玉米 株高 数量性状基因座位 贝叶斯统计 R qtlbim 

分 类 号：S513[农业科学—作物学]