β-溶血型链球菌与角质形成细胞增殖和凋亡研究  

Study on the Interrelation between Streptococci and Proliferation or Apoptosis of Keratinocytes

作　　者：张峻岭[1] 陈学荣[2] 殷金珠[3] 

机构地区：[1]天津市长征医院皮肤科,天津300021 [2]北京医科大学第三临床医院皮肤科 [3]北京医科大学免疫室

出　　处：《中国皮肤性病学杂志》2000年第5期302-304,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的　探讨 β 溶血型链球菌诱发银屑病的机制。 方法　3H TdR掺入法、流式细胞仪测定亚二倍体含量、片段化DNA分析、AnnexinV凋亡检测法。结果　链球菌悬液活化的外周血淋巴细胞培养上清液作用于角质形成细胞 48h ,可促进角质形成细胞增殖 ,再次加入上清液继续作用 48h ,则诱导细胞凋亡 (P <0 .0 1 ) ;而链球菌本身对角质形成细胞增殖或凋亡无明显影响。结论　链球菌作为超抗原活化T细胞 ,使之释放细胞因子而使角质形成细胞首先增殖 。Objective To investigate the relationship between streptococci and pathogenesis of psoriasis.Methods 3H TdR incorporation assay,flow cyteometic analysis quatification of hypodiploid cell,analysis of DNA fragmentation,AnnexinV method.Results the supernatants of periphral blood mononuclear cells(PBMCs)stimulated by streptococci suspesion prompted proliferation of keratinocytes after 48hrs,then it induced apoptosis of keratinocytes after the supernatants had been added once again for another 48hrs( P <0.01);streptococci suspesion itself had no direct role to proliferation and apoptosis of keratinocytes.Conclusion Streptococcal superantigen actived T cells,lead to the release of cytokines,which prompted keratinocytes to proliferation then apoptosis,resulted in psoriasis.

关 键 词：链球菌 超抗原 银屑病 角质形成细胞 细胞增殖 

分 类 号：R758.630.2[医药卫生—皮肤病学与性病学]