复方参芩对防止心肌细胞免受犬细小病毒损伤的保护和抗氧化作用  

Antioxidation Effects of Compound Shenqin to Protect the Myocardial Cells from Canine Parvovirus-Caused Injuries

作　　者：桂志胜[1] 刘娟[1,2] 杜林林[1] 凌榕镔[1] 郭志兴[1] 

机构地区：[1]西南大学(荣昌校区)动物医学系,重庆荣昌402460 [2]重庆市药效评价工程技术研究中心,重庆荣昌402460

出　　处：《西南大学学报（自然科学版）》2013年第7期27-31,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(31172357)

摘　　要：目的:探讨复方参芩防止心肌细胞免受犬细小病毒损伤的保护和抗氧化作用.方法:培养的乳鼠心肌细胞,加入犬细小病毒液致心肌细胞病毒性氧化损伤,测定乳鼠心肌细胞裂解液中SOD,MDA及用MTT法测定细胞活性,探究复方参芩防止心肌细胞免受犬细小病毒损伤的保护和抗氧化作用.结果:病毒损伤组细胞保护率为70.67%±1.33%,复方参芩高、中、低剂量组细胞保护率分别为83.52%,92.35%,80.72%,与病毒损伤组相比有极显著性差异(p<0.01);病毒损伤组细胞裂解液中SOD活性降低,MDA质量分数增高,而复方参芩组上述指标有所改善,其中SOD活性升高,MDA降低,与模型组相比差异极显著(p<0.01).结论:复方参芩可防止心肌细胞免受犬细小病毒损伤,其机理可能与增强细胞抗氧化作用、减少自由基和脂质过氧化导致的细胞损伤有关.Aims： To investigate the anti-oxidative role of compound Shenqin in protecting neonatal rat cardiomyocytes from canine parvovirus-caused injuries.Methods： Neonatal rat cardiomyocytes were infected by the canine parvovirus to induce viral oxidative injury.The cell livability was determined by the amounts of SOD and MDA as well as by MTT assay.Results： The protection of the control group was 70.67%±1.33.The protections of the experimental groups with high,moderate and low dosage of compound Shenqin injection were 83.52%,92.35% and 80.72%,respectively.Also,a significant difference of the cell livability was observed between the experimental groups and the control group,p0.01.In addition,the viral injured cell lysis solution showed a low level of SOD and high concentration of MDA.On the contrary,the experimental group improved SOD level and reduced MDA secretion,p0.01.Conclusion： Compound Shenqin protects the canine parvovirus-infected neonatal rat cardiomyocytes.This might be related to the enhanced cell anti-oxidability and the reduced free radicals and overoxidative lipids causing cell injury.

关 键 词：复方参芩 心肌细胞 抗氧化 

分 类 号：Q939.46[生物学—微生物学]