不同储存年份的艾条燃烧生成自由基的ESR波谱研究  

A study on ESR Spectrum of the Free Radicals Produced by Burning Moxa Stick in Different Years

作　　者：窦传字[1] 吴焕淦[1] 洪宗国[2] 崔云华[1] 周次利[1] 马晓芃[1] 刘慧荣[1] 吕丰[2] 郭金云[2] 

机构地区：[1]上海中医药大学上海市针灸经络研究所,上海200030 [2]中南民族大学药学院,武汉430074

出　　处：《世界中医药》2013年第8期852-855,共4页World Chinese Medicine

基　　金：国家重点基础研究发展计划(编号:2009CB522900);上海市卫生局科研项目资助;国家自然科学基金(编号:81102637)

摘　　要：目的:研究艾条燃烧生成自由基种类及其含量随储存期的变化,从自由基角度探讨新、陈艾的安全性。方法:采用电子自旋共振(ESR)技术,对艾绒产地为湖北蕲春的1年、3年、5年储存期的艾条燃烧生成的自由基进行检测和分析。结果:1)储存期分别为1年、3年、5年的艾条燃烧产生的焦油和烟气中均检测到自由基信号,检测到的焦油自由基以醌/半醌自由基为主,气相自由基包括烷基、烷氧自由基和NO自由基。2)储存期为3年、5年的艾条燃烧生成醌/半醌自由基、烷类和烷氧自由基以及NO自由基的信号均低于1年艾条(P<0.05,P<0.01),而储存期为3年和5年的艾条燃烧生成的醌/半醌自由基、烷类和烷氧自由基信号比较无统计学意义(P>0.05),5年艾条燃烧生成的NO自由基信号强度低于3年艾条(P<0.01)。结论:艾条燃烧生成的自由基主要为醌/半醌自由基、烷基和烷氧自由基以及NO自由基,储存期为3年和5年艾条燃烧生成的上述自由基均少于1年艾条;艾烟中自由基的检测有可能成为新陈艾鉴别和艾灸安全性评价的重要依据。Objective：To observe the changes of species and contents of free radicals generated by burning moxa with different storage time,and discuss the security of moxa from free radicals perspective.Methods：Detected and analysed free radicals in burning Qichun moxa sticks with 1 year,3 years,5 years storage time respectively using electron spin resonance （ESR） technique.Results：1） Free radicals were detected in both tar and gas phase of burning moxa,mainly quinone/semiquinone radicals in tar and alkyl,alkoxy,NO radicals in gas;2） the signal intensity of all detected free radicals in burning moxa with 3 years and 5 years storage time was inferior to it in 1 year moxa（P 0.05,P 0.01）,quinone/semiquinone radicals,alkyl and alkoxy radicals had no difference between burning 3 years moxa sticks and 5 years moxa sticks （P 0.05）,while the signal intensity of NO radicals in 5 years moxa was under that in 3 years moxa（P 0.01） .Conclusion：The free radicals generated from burning moxa mainly were quinone/semiquinone radicals,alkyl and alkoxy radicals,and NO radicals,free radicals mentioned above were less in burning moxa with 3 years and 5 years storage time than that in 1 year storage time moxa.The detection of free radicals might become an important evaluation basis for identification of moxa with different storage time and moxibustion security authentication.

关 键 词：艾灸生成物 自由基 电子自旋共振(ESR) 生物活性 安全性 

分 类 号：R979.1[医药卫生—药品]