胰岛素样生长因子-Ⅱ对培养人牙周膜成纤维细胞生物学活性的影响  

The effect of insulin-like factor-Ⅱ on the biological activities of cultured human

作　　者：冯雪[1] 林珠[1] 段银钟[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2000年第5期265-267,共3页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金!(3980 0 0 33)

摘　　要：目的 :研究IGF -Ⅱ对培养人牙周膜成纤维细胞 (PDLF)生物学活性的影响。方法 :接种一定量培养至第 5代的人PDLF ,用MTT法和碱性磷酸酶比色法观察IGF -Ⅱ作用下 ,PDLF的增殖和碱性磷酸酶活性的变化。结果 :IGF -Ⅱ在实验浓度范围内 ,对PDLF有较明显的促增殖作用 ,对PDLF的ALP活性具有较强的促进作用。结论 :IGF -Ⅱ对PDLF的生物学活性有一定的影响 。AIM: To investigate the effect of Insulin-like factor- Ⅱ(IGF-Ⅱ)on the biological activities of cultured human periodontal ligament fibroblasts(PDLF). METHODS: Seeding certain concentration of the fifth generation of cultured PDLF into 96 plates, to determine proliferation and the activity of alkaline phosphatase(ALP) using MTT method and ALP kit respectively after the input of IGF-Ⅱ. RESULT: IGF-Ⅱcould enhance both of proliferation and ALP activity within the experimental concentration. CONCLUSION: IGF-Ⅱcould influence the biological activities of PDLF,and it might modulate the remodeling process of peridontal tissue through the function of PDLF [

关 键 词：胰岛素样生长因子-II 牙周膜成纤维细胞 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学] R329.3[医药卫生—基础医学]