贵州省山羊流产主要病原菌分离鉴定及致病性试验  

Identify and pathogenicity test of major pathogenic bacteria from goat abortion in Guizhou province

作　　者：罗意[1] 周碧君[1] 李涛 张元鑫 张华 王开功[1] 文明[1] 程振涛[1] 李世静[1] 

机构地区：[1]贵州大学动物科学学院/贵州省动物疫病研究室,贵州贵阳550025 [2]贵州省农业委员会,贵州贵阳550001

出　　处：《广东农业科学》2013年第15期134-136,148,共4页Guangdong Agricultural Sciences

基　　金：贵州省农业科技攻关项目(黔科合NY字[2011-3105]号);黔西南州种草养羊产业发展省州科技合作专项(黔西南科合[2012]5号);贵州省农业科技攻关项目(黔科合NY字[2011]3061号);贵州省重大科技专项计划项目(黔科NZ字[2011]3005号)

摘　　要：研究了贵州省山羊流产主要病原菌的组成、分布和致病情况,为贵州省山羊流产细菌的防控提供理论参考。对贵州省6个地(州、市)10个规模养羊场91只流产山羊的胎儿、子宫与母羊的阴道棉拭子进行细菌样本的采集并分离,通过革兰氏染色和生化试验鉴定,并对山羊阴道棉拭子中分离鉴定的4种主要优势菌株作小白鼠致病性试验。在贵州省山羊生殖系统中共分离到13种(科、属)细菌共计159株,其中在流产山羊中分离到的细菌种类显著多于健康山羊;4种主要优势菌株对小鼠均存在一定致病性,对小鼠致死率高低依次为溶血巴氏杆菌、化脓链球菌、腐生葡萄球菌和大肠埃希氏菌,分别为83.3%、50%、8.3%和8.3%。To provide information on the prevention and control of goat abortion bacteria for Guizhou province,this article studied the composition,distribution and pathogenicity of major pathogenic bacteria of goat abortion in Guizhou province.The bacteria samples were collected and separated from fetus,uterus,ewe vaginal cotton swab of 91 abortion goats from 10 scale sheep farms in 6 prefectural（state,city） of Guizhou province,identification of bacterium by the gram staining and biochemical tests were conducted,then the pathogenicity test in mice was conducted with the four major advantage bacteria.13 species bacteria,159 strains in total,were isolated from the reproductive system of goat in Guizhou province,the bacterial species isolated from abortion goats were significantly more than the healthy goats.The 4 major bacterium existed certain pathogenic to mice,the order of the fatality rates in mice were Pasteurella haemolytica,Streptococcus pyogenes,Staphylococcus saprophyticus and Escherichia coli,the values were 83.3%,50%,8.3% and 8.3%,respectively.

关 键 词：山羊流产 主要病原菌 分离鉴定 致病性试验 

分 类 号：S852.61[农业科学—基础兽医学]