刺五加GAPDH基因全长cDNA的克隆与生物信息学分析  

Cloning and bioinformatics analysis of cDNA of GAPDH gene from Eleutherococcus senticosus

作　　者：修乐山[1] 柴丽花[1] 周秘[1] 邢朝斌[1] 

机构地区：[1]河北联合大学生命科学学院,河北唐山063000

出　　处：《广东农业科学》2013年第16期124-127,共4页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(30701086);河北省自然科学基金(C2009001252);河北省自然科学基金-石药集团医药联合研究基金(H2012401006)

摘　　要：采用cDNA末端RACE技术,从刺五加叶片中克隆到GAPDH基因cDNA的全长序列,并运用生物信息学方法对该基因的cDNA序列、保守区、氨基酸序列、亲缘关系等特征进行分析,并预测其编码蛋白质的结构和功能。研究结果表明,刺五加GAPGH基因的cDNA全长为1 437 bp,开放阅读框全长为1 023 bp,编码一个具有340个氨基酸残基的蛋白,蛋白分子质量为37.070 ku,理论等电点(pI)为7.71。刺五加GAPDH蛋白不存在跨膜结构域,定位于细胞质中,属于亲水蛋白。GAPDH gene full length cDNA was cloned by RACE from leaves of Eleutherococcus senticosus.Some characters involving in cDNA sequence,amino acid sequence,conservative domain and relationship were analyzed by bioinformatics method,and the structure and function of GAPDH gene were deduced.The cDNA of GAPDH gene from E.senticosus was successfully cloned.The full length of GAPDH gene was 1 437 bp containing a 1 023 bp open reading frame（ORF） that encoded a protein with 340 amino-acid residues,the relative molecular mass of GAPDH protein of E.senticosus was 37.070 ku and the isolectric point was 7.71.Without transmembrane domain,GAPDH protein of E.senticosus was located in cytroplasm,and belong to hydrophilic protein.The result has made foundation for analysis the key enzyme gene of express variance of E.senticosus in transcription level.

关 键 词：刺五加 GAPDH 克隆 

分 类 号：Q785[生物学—分子生物学]