肺泡Ⅱ型上皮细胞染色体脆性位点基因FHIT与肺间质纤维化的关系  

Relationship between fragile sites gene of FHIT in type Ⅱ pneumocytes and idiopathic pulmonary fibrosis

作　　者：谷雨[1] 段斐[2] 党志勇[2] 孙晓芳[2] 李少春[2] 薛娟[2] 刘未华[2] 郝华超[2] 李锦 

机构地区：[1]河北大学,附属医院药学部,保定071000 [2]河北大学基础医学院,保定071000 [3]秦皇岛市中医院,秦皇岛066002

出　　处：《解剖学杂志》2013年第4期720-723,共4页Chinese Journal of Anatomy

基　　金：河北省留学人员科技活动项目基金（20100306）

摘　　要：目的：研究肺间质纤维化模型大鼠肺泡Ⅱ型上皮细胞染色体脆性位点基因FHIT表达的改变.方法：SD雄性大鼠随机分为假手术组、模型组及川芎嗪用药组,分别于给药7、14、28 d后提取肺泡Ⅱ型上皮细胞行体外培养,RT-PCR法检测FHIT基因mRNA表达的改变,免疫组织化学检测FHIT蛋白表达.结果：与假手术组比较,造模7d后模型组大鼠肺泡Ⅱ型上皮细胞染色体脆性位点基因FHIT mRNA及蛋白表达无变化.用药14、28 d后,FHIT基因mRNA及蛋白表达则明显下降.而川芎嗪组比模型组有显著上调.结论：在肺间质纤维化过程中存在染色体脆性位点基因FHIT的改变,川芎嗪可以一定程度上抑制这种改变从而起到防治作用.Objective.. To investigate the changes in expression of FHIT, a fragile sites gene, in type Ⅱ pneumocytes isolated from rats with idiopathic pulmonary fibrosis. Methods~ SD rats were randomly divided into 3 groups （n= 6 in each group） ： a sham-operated group, a model group and a tetramethylpyrazine group. After given bleomycin, the rats were harvested at 7, 14 and 28 days to isolate the type Ⅱ pneumocytes and then the cells were cultured in vitro. Then, we detected the protein expression of FHIT in pneumocytes with immunocytochemistry and its mRNA expression with RT-PCR. Results： Compared with the sham-operated group, no significant differences in FHIT expression were found in rat type ]I pneumocytes of the model group administrated with bleomycin for 7 days. After 14 and 28 days of administration, the FHIT mRNA and protein expression was redueed greatly in the model group. Nevertheless, tetramethylpyrazine significantly enhaneed FHIT expression at both mRNA and protein levels compared to the model group. Conclusion： In this study, we demonstrate that the expression of fragile sites gene of FHIT is altered during idiopathic pulmonary fibrosis, while tetramethylpyrazine suppresses the alteration to some extent, suggesting that the medication could play a protective role for idiopathic pulmonary fibrosis.

关 键 词：肺间质纤维化 肺泡Ⅱ型上皮细胞 川芎嗪 染色体脆性位点基因FHIT 

分 类 号：R336[医药卫生—人体生理学]