基于聚合酶链式反应列阵技术对甘肃省儿童医院新生儿B族链球菌感染的调查研究  

Neonatal group B streptococcus infection in the Children＇s Hospital of Gansu Province through PCR array

作　　者：杨静[1] 徐丁[1] 尹立琴[1] 朱保权[1] 王爱华[1] 

机构地区：[1]兰州大学第二医院甘肃省儿童医院小儿内科,730000

出　　处：《中华儿科杂志》2013年第9期688-691,共4页Chinese Journal of Pediatrics

基　　金：甘肃省自然科学基金（096RJZA067）

摘　　要：目的通过聚合酶链式反应（PCR）列阵技术对甘肃省儿童医院新生儿B族链球菌（GBS）感染情况进行调查研究。方法在监护人知情同意下，收集2011年6月至2012年1月甘肃省儿童医院住院的有感染症状的新生儿外周血标本286份。采用5％Chelex-100＋0．5％NP40提取DNA，利用NCBI数据库优选25个GBS相关基因，并使用该数据库primer．BLAST工具分别设计25对引物。通过阳性菌株对每对引物进行PCR条件优化，然后对25对引物进行列阵，对所有样本进行PCR列阵分析。结果共检测到阳性标本14份，占人选标本的4．90％。≤7d以内新生儿阳性率为4．55％，〉7d新生儿阳性率为5．19％，早产儿53例，阳性率为5．66％。入选标本随访调查，无一例病死。结论甘肃省儿童医院新生儿感染性疾病中GBS感染率为4．90％。PCR列阵分析表明，免疫逃避相关基因的表达频率相对较高，提示在感染过程中，免疫适应可能是GBS采取的主要致病策略。Objective To study neonatal Streptococcus agalactiae （GBS） infection in The Children＇s Hospital of Gansu Province through Polymerase Chain Reaction （PCR） Array. Method After obtaining the imformed consent from parents or guardians, blood samples of 286 neonates were collected and studied in The Children＇s Hospital of Gansu Province from June 2011 to January 2012. DNA of the selected samples was extracted through the method of 5% Chelex-100 ＋ 0. 5% NP40 solution. Twenty-five genes were ultimately selected and then 25 pairs of primers were designed respectively through primer-BLAST tool of NCBI database. For every primer,PCR conditions were optimized through the identified GBS,and 25 pairs of primers were arrayed as to be used to study neonatal GBS infection. Result The results of PCR Array showed that the 14 samples were detected positive, accounting for 4. 90% of all the selected specimens. As for neonatal GBS infection,the positive rate was 4. 55% within 7 days after birth and 5.19% in those older than 7 days. The positive rate of 53 preterm infants was 5.66%. The follow-up survey showed that none of the cases died. Conclusion In the Children＇s Hospital of Gansu Province neonatal GBS infection rate was 4. 90%, which is similar to the previous domestic reports, but is lower than the reports from Europe and the United States. Studies have shown that the gene expression related to immune evasion has a higher frequency. The present study suggests that the strategy of GBS immune adaptation may play an important role in neonatal GBS infection.

关 键 词：链球菌 无乳 聚合酶链反应 婴儿 新生 感染 

分 类 号：R440[医药卫生—诊断学]