适于脑胶质瘤浸润MRI研究的动物模型构建  

Establishment of Rat Glioma Model Suitable for MRI Study of Glioma Infiltration

作　　者：魏晓[1] 李香营[1] 袁园[1] 陈晶[1] 黄邓高[2] 谈顺[3] 韩向君[1] 

机构地区：[1]中南大学湘雅医学院附属海口医院/海口市人民医院放射科 [2]中南大学湘雅医学院附属海口医院,海口市人民医院中心实验室 [3]中南大学湘雅医学院附属海口医院/海口市人民医院病理科

出　　处：《中国医学计算机成像杂志》2013年第4期390-392,共3页Chinese Computed Medical Imaging

基　　金：海南省卫生厅项目(琼卫2010-61),海南省卫生厅项目(琼卫2011-67);海南省自然科学基金(813259);海口市重点科技计划项目(No 2011-0145)~~

摘　　要：目的：建立适用于MRI瘤周浸润研究的鼠脑胶质瘤模型。方法：通过立体定向技术，将c6细胞接种至A、B两组大鼠右侧尾状核，对照组在相同部位注射全培养基。A、B两组荷瘤大鼠分别于接种后1～2和3～4周利用3．0T磁共振扫描仪行常规MRI、T1wI增强扫描。病理观察肿瘤级别及肿瘤浸润程度，分析A、B两组肿瘤细胞浸润程度差别。结果：49只存活的细胞接种大鼠，在随后的MRI或病理检查中证实有肿瘤形成，对照组大鼠0=5）未见肿瘤形成。磁共振成像显示B组瘤体较A组体积大，信号欠均匀，增强后呈明显均匀强化或环状强化。A、B组荷瘤大鼠瘤周浸润程度分别平均为（0．083士0．005）和（0．315±0．012），两组结果经两样本，检验显示差别有统计学意义（t=11．8，P〈0．05）。结论：肿瘤细胞接种后3～4周是肿瘤细胞浸润最明显的阶段，可作为MRI脑胶质瘤浸润研究的理想模型。Purpose： To establish the stable and reliable rat brain glioma model for MRI study of glioma peritumoral invasion. Methods： C6 glioma cells and whole culture medium were stereotactically injected into the right caudate nucleus of the experimental group （group A, B） and control group, respectively. About 1-2 weeks and 3-4 weeks after implantation, all survival rats were examined by conventional MRI, enhanced TIWI scans using a GE 3.0T MRI scanner, respectively. Tumor grade and degree of tumor infiltration were observed under the microscope. And difference of tumor infiltration degree was analyzed between group A and B. Results： Forty-nine survival rats were confirmed with the tumor formation by the subsequent MRI scans or pathological examination. And rats in control group （n=5） were with no tumor formation. Magnetic resonance imaging showed that tumor volumes of rat brain glioma were larger in group B than that in group A, and the tumors were characterized by inhomogeneous signal and homogeneous enhancement or ring enhancement. Degree of tumor infiltration in group A and B was （0.083±0.005） and （0.315±0.012）, respectively. The difference was with statistical significant between group A and B （t=11.8, P〈0.05）. Conclusion： The rat brain glioma model is in the most significant stage of tumor cell infiltration on about 3 to 4 weeks after inoculation, and it can be used as the ideal model for MRI study of brain glioma infiltration.

关 键 词：胶质瘤 动物模型 磁共振成像 瘤周浸润 

分 类 号：R445.2[医药卫生—影像医学与核医学]