Decreased and dysfunctional circulating endothelial progenitor cells in patients with chronic obstructive pulmonary disease  

作　　者：GAN Ye CAO Jun CHEN Yan HE Zhi-hui LUO Hong CAI Shan XIANG Xu-dong ZHOU Rui CHEN Ping YANG Yue 

机构地区：[1]Department of Geriatrics , Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China [2]Departement of Rehabilitation Medicine, Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China [3]Department of Respiratory Medicine, Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China [4]Department of Intensive Care Unit, Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China [5]Department of Emergency Medicine , Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China [6]Department of Respiratory Medicine, Hunan Provincial People's Hospital, Changsha, 410005, China

出　　处：《Chinese Medical Journal》2013年第17期3222-3227,共6页中华医学杂志（英文版）

基　　金：This work was supported by grants from National Natural Science Foundation of China （No. 81070039, No. 81270100 and No. 81200026） and from the Natural Science Foundation of Hunan Province （No. 2008FJ3152 and No. 2011 SK3237）.

摘　　要：Background It has been widely demonstrated that endothelial progenitor cells are involved in several diseases and that they have therapeutic implications. In order to define the altered pulmonary vascular homeostasis in chronic obstructive pulmonary disease, we sought to observe the level and functions of circulating endothelial progenitor calls in patients with chronic obstructive pulmonary disease. Methods The total study population included 20 patients with chronic obstructive pulmonary disease and 20 control subjects. The number of circulating endothelial progenitor cells （CD34＋/CD133＋/IVEGFR-2＋cells） was counted by flow cytometry. Circulating endothelial progenitor cells were also cultured in vitro and characterized by uptake of Dil- acLDL, combining with UEA-I, and expression of von Willebrand factor and endothelial nitric oxide synthase. Adhesion, proliferation, production of nitric oxide, and expression of endothelial nitric oxide synthase and phosphorylated-endothelial nitric oxide synthase were detected to determine functions of circulating endothelial progenitor cells in patients with chronic obstructive pulmonary disease. Results The number of circulating endothelial progenitor cells in the chronic obstructive pulmonary disease group was lower than in the control group： （0.54±0.16）% vs. （1.15±0.57）%, P 〈0.05. About 80% of adherent peripheral blood mononuclear cells cultured in vitro were double labeled with Dil-acLDL and UEA-I. The 92% and 91% of them were positive for von Willebrand factor and endothelial nitric oxide synthase, respectively. Compared with the control, there were significantly fewer adhering endothelial progenitor cells in chronic obstructive pulmonary disease patients： 18.7±4.8/field vs. 45.0±5.9/field, P 〈0.05. The proliferation assay showed that the proliferative capacity of circulating endothelial progenitor cells from chronic obstructive pulmonary disease patients was significantly impaired： 0.135±0.038 vs. 0.224±0.042, P 〈0.05. Furthermore, niBackground It has been widely demonstrated that endothelial progenitor cells are involved in several diseases and that they have therapeutic implications. In order to define the altered pulmonary vascular homeostasis in chronic obstructive pulmonary disease, we sought to observe the level and functions of circulating endothelial progenitor calls in patients with chronic obstructive pulmonary disease. Methods The total study population included 20 patients with chronic obstructive pulmonary disease and 20 control subjects. The number of circulating endothelial progenitor cells （CD34＋/CD133＋/IVEGFR-2＋cells） was counted by flow cytometry. Circulating endothelial progenitor cells were also cultured in vitro and characterized by uptake of Dil- acLDL, combining with UEA-I, and expression of von Willebrand factor and endothelial nitric oxide synthase. Adhesion, proliferation, production of nitric oxide, and expression of endothelial nitric oxide synthase and phosphorylated-endothelial nitric oxide synthase were detected to determine functions of circulating endothelial progenitor cells in patients with chronic obstructive pulmonary disease. Results The number of circulating endothelial progenitor cells in the chronic obstructive pulmonary disease group was lower than in the control group： （0.54±0.16）% vs. （1.15±0.57）%, P 〈0.05. About 80% of adherent peripheral blood mononuclear cells cultured in vitro were double labeled with Dil-acLDL and UEA-I. The 92% and 91% of them were positive for von Willebrand factor and endothelial nitric oxide synthase, respectively. Compared with the control, there were significantly fewer adhering endothelial progenitor cells in chronic obstructive pulmonary disease patients： 18.7±4.8/field vs. 45.0±5.9/field, P 〈0.05. The proliferation assay showed that the proliferative capacity of circulating endothelial progenitor cells from chronic obstructive pulmonary disease patients was significantly impaired： 0.135±0.038 vs. 0.224±0.042, P 〈0.05. Furthermore, ni

关 键 词：circulating endothelial progenitor cells chronic obstructive pulmonary disease endothelial nitric oxide synthase nitric oxide 

分 类 号：Q51[生物学—生物化学] Q462