过表达小鼠UNCV蛋白质对HeLa细胞凋亡的影响  

Effects of overexpressed mouse UNCV protein on apoptosis in HeLa cells

作　　者：刘冰[1] 徐嫄[1] 赵爽[1] 王冬平[1] 杨蕾蕾[2] 李微[1] 尚世臣[1] 张广州[1] 李文龙[1] 曾林[1] 

机构地区：[1]军事医学科学院实验动物中心,北京10071 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中国实验动物学报》2013年第4期6-9,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然基金重点项目资助(31030058)

摘　　要：目的研究小鼠UNCV蛋白质对HeLa细胞凋亡的影响。方法将BALB/c小鼠Uncv基因重组质粒转染HeLa细胞,筛选出稳定过表达UNCV蛋白质的HeLa细胞株。通过细胞计数法和流式细胞术,检测过表达UNCV蛋白质对血清饥饿和阿霉素诱导的HeLa细胞凋亡的影响。结果获得稳定正确过表达UNCV蛋白质的HeLa细胞株。细胞计数和流式细胞术结果显示过表达UNCV蛋白质对血清饥饿诱导的HeLa细胞凋亡有抑制作用,对于阿霉素诱导的HeLa细胞凋亡没有显著影响。结论过表达UNCV蛋白质对血清饥饿诱导的HeLa细胞凋亡有抑制作用。Objective To study the effects of mouse UNCV protein on apoptosis in HeLa cells. Methods The recombinant plasmids containing Uncv gene of BALB/C mice were transfected into HeLa cells. The HeLa cells stably over- expressing UNCV protein were selected. The effects of overexpressed UNCV protein on apoptosis in HeLa cells induced by doxorubicin and serum starvation were measured by cell counting and flow cytometry. Results HeLa cells stably overex- pressing UNCV protein were obtained. The results of cell counting and flow cytometry showed that the overexpression of UNCV protein inhibited apoptosis in HeLa cells induced by serum starvation. The effects of UNCV protein on apoptosis in HeLa cells induced by doxorubicin were not significantly different. Conclusions Overcxpression of UNCV protein inhibits apoptosis in HeLa cells induced by serum starvation.

关 键 词：UNCV 细胞凋亡 血清饥饿 阿霉素 HELA细胞 

分 类 号：Q95-33[生物学—动物学]