MicroPET观察姜黄素对9月龄AD小鼠脑葡萄糖代谢的影响  

MicroPET observation of the effect of curcumin on cerebral glucose metabolism in 9-month old double transgenic mice with Alzheimer’s disease

作　　者：冯慧利[1,2] 高凯[3] 魏鹏[1,2] 王虹[4] 任映[1,2] 孙海芸[1,2] 杨金铎[1,2] 王蓬文[1,2] 

机构地区：[1]国家中医药管理局三级实验室北京中医药大学东直门医院中药药理学实验室,北京100700 [2]北京中医药大学东直门医院中医内科学教育部重点实验室和北京市重点实验室,北京100700 [3]中国医学科学院医学实验动物研究所,北京100021 [4]北京美中宜和妇儿医院,北京100016

出　　处：《中国实验动物学报》2013年第4期38-41,F0003,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然基金面上资助项目(No.81073076);教育部高等学校创新团队(IRT0810)

摘　　要：目的通过氟18-脱氧葡萄糖MicroPET(18F-FDG microPET)影像技术观察姜黄素对9月龄AD小鼠的脑葡萄糖代谢影响。方法随机挑选9月龄(3月龄小鼠,干预6个月)模型组、西药组和姜黄素大、中、小剂量组AD小鼠各3只,以及同月龄C57BL/6J正常对照组小鼠3只,通过吸入2%异氟烷麻醉后,每例弹丸式从尾静脉注射放射性示踪剂18F-FDG约14.8～16.5 MBq,摄取1 h后采集10 min MicroPET图像。计算每组小鼠每克脑组织(除小脑)18F-FDG的摄取率并比较分析其差异。结果姜黄素中剂量组小鼠和西药组小鼠每克脑组织18F-FDG的摄取率高于正常对照组和模型组。结论姜黄素治疗后,9月龄AD模型小鼠脑18F-FDG的摄取有所改善,提示AD模型脑糖代谢所反映的脑功能得到改善,姜黄素发挥了神经保护作用。Objective To observe the effect of curcumin on the cerebral glucose metabolic status in 9-month old APP/PS1 double transgenic mice by 1SF-FDG microPET. Methods Three-month-old APP /PSI double transgenic mice were randomly divided into the mode] group, positive rosiglitazonc control group and curcumin high-dose （400 mg/kg, d） , medium-dose （200 mg/kg.d） and low-dose （100 mg/kg.d） groups, with normal C57BL/6J mice as normal control group （ n = 3 ） for this microPET study. At 6 months after the drug administration, the mice were anesthetized using 2% isoflurane and injected through the tail vein 14. 8 - 16. 5 MBq ISF-FDG as a bolus. PET scans were performed for 10-min at one hour after the 1SF- FDG uptake. The 1SF-FDG uptake rate per gram tissue of the whole brain （ except cerebellum） was calculated and compared among the mice of different groups. Results The cerebral 18F-FDG uptake rate per gram tissue in the mice of medium-dose curcumin group and positive rosiglitazone control group were higher than that in both the normal control and model groups. Conclusions Curcumin increases the cerebral 1SF-FDG uptake and SF-FDG uptake rate per gram tissue in the APP/PS1 double transgenic mice, and may play a neuroprotective role through improving cerebral glucose metabolism.

关 键 词：脑葡萄糖代谢 姜黄素 APP/PS1双转基因小鼠 氟18-脱氧葡萄糖 

分 类 号：Q95-33[生物学—动物学] R285.5[医药卫生—中药学]