光滑念珠菌Cdc42基因生物信息分析  

Bioinformatics Analysis of Cdc42 Gene from Candida Glabrata

作　　者：赵静[1] 黄怀球[1] 袁立燕[1] 钟毅[2] 张静[3] 张晓辉 

机构地区：[1]中山大学附属第三医院皮肤性病科,广东广州510630 [2]广东省第二中医院,广东广州510095 [3]中山大学附属孙逸仙纪念医院皮肤性病科,广东广州510120 [4]广东省皮肤病医院,广东广州510091

出　　处：《皮肤性病诊疗学杂志》2013年第4期234-237,共4页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：广东省自然科学基金(编号:S2012020011056)

摘　　要：目的:分析和预测光滑念珠菌Cdc42基因及其编码蛋白的结构和特性。方法:利用NCBI、ExPASy和CBS网站中的各种信息分析工具,并结合Vector NTI suite 8.0生物信息学分析软件包,分析预测光滑念珠菌Cdc42基因并预测该基因编码蛋白结构的特征和功能。结果:Cdc42基因全长为576 bp,编码区具有191个氨基酸,在GenBank同源序列中,其与酵母Cdc42氨基酸序列一致性达到99%,且有Cdc42保守域。Cdc42蛋白相对分子量预测为21 420.83,理论等电点为6.31。预测Cdc42编码蛋白ɑ螺旋(H)、β折叠(E)、无规则卷(L)的比例分别是29.84%、28.70%、41.88%,1个GTP/ATP结合位点。Cdc42蛋白为疏水蛋白,无跨膜区,无信号肽。结论:成功预测Cdc42基因及编码蛋白生化及结构特征,为下一步对其进行克隆和表达奠定基础。Objective：To analyze and predict the structure and properties about encoding pro- tein of cell division cycle 42 （Cdc42） from Candida glabrata by bioinformatics. Methods： A full- length cDNA sequence encoding Cdc42 from Candida glabrata was identified by using tools of bioinformatics at webs sites of NCBI, ExPASy, CBS and software Vector NTI suite 8.0. The char- acteristics of the protein were predicted by employing bioinformatics software package supplied by the website of ExPaSy. Results：The full length of Cdc42 is 576 bp, and its ORF encodes 191 ami- no acid. The relationship of phylogenesis between Candida glabrata and other fungus is close. The prediction shows that Cdc42 had a Cdc42 conserved domain, the molecular weight and theoretical pI of Cg. Cdc42 was 21 420. 83 and 6.31 respectively, and the coding protein contains 29. 84% a-helix, 28.70% extended strand,41.88% random coil, and one GTP/ATP motif. Cdc42 enco- ding protein is hydrophobic, extra-membrane protein, without signal peptide. Conclusion： The structure and characteristics of the gene and protein of Cg. Cdc42 was predicted.

关 键 词：光滑念珠菌 细胞分裂周期蛋白(Cdc42) 生物信息学 

分 类 号：R756.5[医药卫生—皮肤病学与性病学]