一种新型活组织材料的组织学及力学特性  

Histological and mechanical properties of a new-type biopsy material

作　　者：陈晓松[1] 张建[2] 李建新[2] 

机构地区：[1]首都医科大学宣武医院泌尿外科,北京市100053 [2]首都医科大学宣武医院血管外科,北京市100053

出　　处：《中国组织工程研究》2013年第34期6110-6115,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家"八六三"重大专项(2006AA02A134);北京市科委重大项目(H020920040330)资助~~

摘　　要：背景:异物诱发包裹产生的包裹组织是一种自体活组织材料,理论上完全避免了非自体植入物所面临的免疫排斥反应和破坏作用,具有合成材料及脱细胞所无法比拟的生物学优势。目的:试图开发出一种只含有自体细胞和细胞外基质成分的自体活组织生物材料,并评价其组织结构及力学特征。方法:将硅胶管植入犬腹腔1个月,获得包裹形成的荚囊样生物组织,然后经过体外修整形成管型结构即自体活组织生物管。对标本进行了力学测定、组织学观察及表面结构分析。结果与结论:腹腔内植入硅胶管形成的自体活组织生物管取材方便,主要由富含Ⅰ型和Ⅲ型胶原的细胞外基质构成,成肌纤维细胞排列在胶原纤维间,呈同心圆样结构,培养1个月时,管壁厚度可达70-250μm,可耐受(1100±187)mmHg(1mmHg=0.133kPa)的静水压,具有很好的可缝合性。提示自体活组织生物管形态可塑性大,由自体细胞及细胞外基质成分构成,避免了免疫排斥反应的发生,且具有足够的力学强度,有希望用于组织工程化血管的体外构建。BACKGROUND： The biopsy material induced by foreign body is autologous tissue, which, in theory, avoids the role of immune rejection and destruction faced by non-autologous implants. This biology advantage has no way to comparison by synthetic and de-cellular materials. OBJECTIVE： To explore a biotube consisting of autologous cells and extracellular matrix and to evaluate its structure and mechanical features. METHODS： The biotubes were prepared by embedding silicon rods as a mold intraperitoneally. Specimens were analyzed by mechanical tests, histological observation and superficial study. RESULTS AND CONCLUSION： It was easy to excite the biotubes prepared by embedding silicon tube intraperitoneally. The biotube mainly included collagen-rich extracellular matrices and myofibroblasts appearing as elongated cells with circumferential or longitudinal orientations. At 1 month of embedding, the wall was 70- 250 pm in thickness and had burst strength of （1 100＋187） mm Hg （1 mm Hg=0.133 kPa）, with perfect sewability. The biotubes, which possess the ability for wide adjustments in their shapes, are composed of autologous cells and extracellular matrices, and are an ideal graft for tissue engineering because they avoid immunological rejection and have sufficient mechanical strength.

关 键 词：生物材料 材料生物相容性 组织工程血管 自体移植 生物管 自体活组织材料 断裂拉伸强度 断裂伸长率 爆破强度 可缝合性 863项目 

分 类 号：R318[医药卫生—生物医学工程]