表面改性聚氨酯平面膜与人下咽成纤维细胞的相容性  

Compatibility of polyurethane membranes after surface modification with human hypopharyngeal fibroblasts

作　　者：康骋[1] 沈志森[2] 陈静静[2] 竺亚斌[1] 

机构地区：[1]宁波大学医学院,浙江省宁波市315211 [2]宁波大学附属李惠利医院耳鼻咽喉-头颈外科,浙江省宁波市315040

出　　处：《中国组织工程研究》2013年第34期6131-6137,共7页Chinese Journal of Tissue Engineering Research

基　　金：宁波市社发择优项目:人工组织瓣构建及其在喉癌下喉癌术后修复中基础与临床应用研究(2012C5015);宁波市科技创新团队-第二层次:咽喉头颈恶性肿瘤诊治创新团队(2012B82019)~~

摘　　要：背景:聚氨酯具有良好的机械物理特性,被广泛应用于医学临床和实验研究,但聚氨酯表面的疏水性使其与组织细胞相容性不理想,从而在一定程度上制约了它作为组织工程中生物材料支架的应用。目的:观察聚氨酯平面膜在接枝丝素蛋白和明胶蛋白后的亲水性及其与人下咽细胞的相容性。方法:采用接触角测试仪分析丝素蛋白或明胶蛋白改性前后聚氨酯平面膜的亲水性能。将体外培养原代人下咽成纤维细胞分别接种于聚氨酯平面膜、丝素蛋白改性聚氨酯平面膜、明胶蛋白改性聚氨酯平面膜及组织培养平板中,采用细胞计数法、细胞形态观察法比较改性前后聚氨酯平面膜的细胞相容性变化。结果与结论:与改性前比较,丝素蛋白或明胶蛋白改性后的聚氨酯平面膜亲水性显著增高(P<0.01),并且丝素蛋白改性聚氨酯平面膜的亲水性高于明胶蛋白改性的聚氨酯平面膜(P<0.01)。在组织培养平板中黏附的细胞数量最多,增殖最快;在丝素蛋白或明胶蛋白改性聚氨酯平面膜中黏附的人下咽成纤维细胞均多于聚氨酯平面膜上的黏附细胞数量,且以丝素蛋白改性聚氨酯平面膜上的细胞较多。表明丝素蛋白或明胶蛋白改性聚氨酯平面膜的亲水性及细胞相容性均有所提高。BACKGROUND： Polyurethane has good mechanical and physical characteristics and is extensively used in clinical and experimental studies, but its hydrophobicity and histocompatibility are not ideal, which limits its use in tissue engineering as a biomaterial scaffold to some extents. OBJECTIVE： To observe the hydrophilicity of polyurethane membrane grafted with silk fibroin and glutin and its compatibility with human hypopharyngeal cells. METHODS： The changes in hydrophilicity of polyurethane membrane grafted with silk fibroin and glutin were detected by contact angle measurements. Human hypopharyngeal fibroblasts were cultured in vitro on polyurethane membrane, silk fibroin-polyurethane membrane, glutin-polyurethane membrane and tissue culture plate. Cell compatibility was compared using cytometry and cell morphology obsevation. RESULTS AND CONCLUSION： Hydrophilicity of silk fibroin- or glutin-polyurethane membranes significantly increased （P 0.01）. The hydrophilicity of silk fibroin-polyurethane membrane was higher than that of glutin-polyurethane membrane （P 0.01）. The number of cells on the tissue culture plate was the most. The number of human hypopharyngeal fibroblasts on the silk fibroin- or glutin-polyurethane membrane was higher than that on the polyurethane membrane, especially on the silk fibroin-polyurethane membrane. These suggested that hydrophilicity and cell compatibility of silk fibroin- or glutin-polyurethane membrane were elev

关 键 词：生物材料 材料生物相容性 聚氨酯 蛋白接枝 丝素蛋白 明胶蛋白 人下咽成纤维细胞 组织工程 

分 类 号：R318[医药卫生—生物医学工程]