皮下植入生肌玉红胶原后的血管新生与瘢痕抑制  

Angiogenesis and scar inhibition after subcutaneous implantation of Shengji Yuhong collagen

作　　者：曹东阳[1] 姚昶[2] 陈德轩[2] 卞卫和[2] 张晓清[2] 尹恒[1] 郭檬檬[1] 

机构地区：[1]南京中医药大学 [2]江苏省中医院,江苏省南京市210029

出　　处：《中国组织工程研究》2013年第34期6144-6151,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30973596);康缘中医药科技创新基金(HZ1009KY);江苏省中医药领军人才基金(LJ2009002)~~

摘　　要：背景:前期研究显示生肌玉红胶原促进血管新生与组织愈合的疗效显著好于生肌玉红膏及单纯胶原或明胶。目的:探察兔皮下植入生肌玉红胶原促进血管新生与修复的疗效与机制。方法:在新西兰兔背部两侧对称制作皮下口袋模型,并对称植入生肌玉红胶原(实验组)与胶原(对照组),于术后3,7,14,28,56d取植入标本及标本周围组织,制作病理切片观察标本周围组织修复状况,测定胶原内血红蛋白水平,免疫荧光与CD34染色标记法观察周围组织微血管新生情况,WesternBlot法检测血管内皮生长因子、血管生成素1的表达,免疫组织化学法观察周围组织Ⅰ、Ⅲ型胶原的分泌及两者的比例。结果与结论:实验组标本周围皮下血管增多,胶原周围组织随着时间推移炎性渗出减少,肉芽组织增生,到28d时已形成成熟的纤维结缔组织,并且促进微血管新生作用及血红蛋白水平高于对照组(P<0.05或P<0.01),术后3,7d血管内皮生长因子、血管生成素1表达高于对照组(P<0.05或P<0.01);实验组Ⅰ型胶原分泌与对照组相当,但术后28,56dⅢ型胶原的分泌高于对照组(P<0.05),且术后28,56dⅠ、Ⅲ型胶原的比例低于对照组(P<0.01)。表明生肌玉红胶原可显著促进周围组织微血管新生,提高血管内皮生长因子与血管生成素1的表达,同时协同调节胶原形成及Ⅰ、Ⅲ型胶原的比例达到减少瘢痕愈合的作用。BACKGROUND： Shengji Yuhong collagen showed good curative effect of promoting angiogenesis and tissue healing compared with Shengji Yuhong Gao and collagen alone or gelatin alone. OBJECTIVE： To explore the curative effect and mechanism of subcutaneous implantation of Shengji Yuhong collagen in rabbits in promoting angiogenesis and repair. METHODS： Shengji Yuhong collagen as the experimental group and collagen as the control group was implanted inside the rabbit subcutaneous pockets of the back of New Zealand rabbits. The implanted samples and surrounding tissues were obtained at 3, 7, 14, 28 and 56 days following surgery. Pathological sections were made and the repair of surrounding tissue was observed. Hemoglobin levels in collagen were measured. Immunofluorescence and CD34 dyeing marking method were utilized to observe capillary angiogenesis. Westernblot assay was employed to examine vascular endothelial growth factor and angiogenin-1 expression. Immunohistochemistry was used to observe the secretion of typeⅠ and Ⅲ collagen on the surrounding tissues. RESULTS AND CONCLUSION： The experimental group showed increased subcutaneous vascularization. There were reduced inflammatory exudation, granulation tissue hyperplasia, and mature fiber connective tissue at 28 days. Angiogenesis and hemoglobin contents were greater in the experimental group than in the control group （P 0.05 or P 0.01）. At 3 and 7 days following surgery, vascular endothelial growth factor and angiogenin-1 expression was greater in the experimental group than that in the control group （P 0.05 or P 0.01）. The secretion of type Ⅰ collagen was identical between the experimental and control groups. However, the secretion of type Ⅲ collagen was higher in the experimental group than in the control group at 28 and 56 days （P 0.05）, and the proportion of type Ⅰ and Ⅲ collagen was lower in the experimental group than in the control group at 28 and 56 days （P 0.01）. These suggested that Shengji Yuhong collagen can significan

关 键 词：生物材料 材料生物相容性 生肌玉红胶原 血管新生 Ⅰ型胶原 Ⅲ型胶原 血管内皮生长因子 血管生成素1 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]