MK-801对改善实验性创伤性脑损伤后脑细胞线粒体呼吸作用的研究  

Effects of MK-801 on the brain mitochondrial respiratory function in experimental traumatic brain injury

作　　者：代文光[1] 秦晓勇[1] 李良慧[1] 宋学良[1] 冯玥[1] 李臻[1] 

机构地区：[1]解放军第二五三医院神经外科,内蒙古呼和浩特010051

出　　处：《创伤外科杂志》2013年第5期436-439,共4页Journal of Traumatic Surgery

摘　　要：目的观察N-甲基-D-天门冬氨酸(N-mechy1 D-aspartate,NMDA)受体拮抗剂(MK-801液)对创伤后大鼠脑线粒体呼吸功能的影响,并探讨可能的作用机制,为临床应用MK-801治疗创伤性颅脑损伤提供依据。方法将Wistar大鼠80只随机分为4组:对照组20只,创伤未治组(非治疗组)20只,治疗24h组(治疗Ⅰ组)20只,治疗72h组(治疗Ⅱ组)20只。采用BIM-Ⅲ型小型多功能生物撞击机造成大鼠中型颅脑损伤,用氧电极法分析线粒体呼吸功能(呼吸Ⅲ、Ⅳ态和呼吸控制率),并行透视电镜观察线粒体超微结构。结果 (1)与对照组比较,非治疗组伤后72h呼吸Ⅲ态和呼吸控制率明显下降;治疗Ⅰ组呼吸功能有所恢复;治疗Ⅱ组呼吸Ⅲ态和呼吸控制率较治疗疗Ⅰ组有显著升高(P<0.05),呼吸Ⅳ态稍有延长,但相差不显著。(2)与对照组比较,电镜结果示伤后非治疗组与治疗Ⅰ组、Ⅱ组线粒体结构均有不同程度的损害,但治疗Ⅰ组、Ⅱ组线粒体损害程度减轻。结论创伤性颅脑损伤后脑线粒体呼吸功能明显下降。应用MK-801治疗颅脑损伤大鼠可明显改善脑线粒体呼吸功能,电镜观察也发现线粒体超微结构损害程度减轻。治疗时间越长,呼吸功能改善越明显。Objective To study the effects of MK-801 on brain mitochondrial respiratory function in rats after experimental traumatic brain injury and the possible mechanisms .Methods A total of 80 Wistar rats were randomly divided into 4 groups：control group with 20 rats, untreated group （ non-traumatic therapy group ） with 20 rats,24 hours treatment group （treatment group 1） with 20 rats,72 hours treatment group （treatment group 2） with 20 rats.The middle degree brain injury in rats was made by BIM-Ⅲmulti-function impacting machine .The brain cell mitochondrial respiratory function was measured with oxygen electrode and the ultrastructure changes were ob -served with transmission electron microscope .Results （1） Compared with the control group ,the brain mitochon-drial respiratory function reduced significantly in the untreated group within 72 hours.Treatment group 1 showed certain degrees of attenuation ,and treatment group 2 showed further improvement .（2） Compared with the control group,the ultrastructural characteristics in the control group remained nomal ,while the other three groups（untreated group,treatment group l and treatment group 2） had different degrees of mitochondrial ultrastructural damage ,which could be attenuated after the treatment of magnesium sulfate .Conclusion The mitochondrial respiratory function decreased significantly after traumatic brain injury .MK-801 could improve the mitochondrial respiratory function in rats after traumatic injury.It could also attenuate the mitochondrial ultrastructural damage .The curative effectis correlated with the course of treatment .The longer the course of treatment ,the better the improvement of mitochon-drial respiratory function .

关 键 词：颅脑损伤 拮抗剂 线粒体 呼吸控制率 大鼠 

分 类 号：R651.15[医药卫生—外科学]