不同脱细胞方法制备的AECM成分分析及比较  

ANALYSIS AND COMPARISON OF THE COMPONENTS OF AECM VIA DIFFERENT DECELLULARIXATION PROTOCOLS

作　　者：贾桦[1] 王莹[2] 佟雷[3] 佟晓杰[3] 

机构地区：[1]宁夏医科大学基础医学院人体解剖与组织胚胎学系,宁夏银川750004 [2]牡丹江医学院解剖教研室,黑龙江牡丹江157011 [3]中国医科大学基础医学院解剖教研室,辽宁沈阳110001

出　　处：《牡丹江医学院学报》2013年第4期1-4,共4页Journal of Mudanjiang Medical University

基　　金：国家自然科学基金(地区项目:81260193);宁夏自然科学基金(NZ12187)

摘　　要：目的:比较不同脱细胞方法制备的大鼠坐骨神经细胞外基质材料(acellular extracellular matrix,AECM)的组分,为解决临床周围神经损伤修复移植体的来源提供实验依据。方法:分别采用优化的化学萃取脱细胞方法和低渗-酶消化法制备大鼠坐骨神经AECM,通过HE染色、变色酸2R-亮绿染色、免疫组织化学染色和SDS-PAGE对AECM的结构成分进行检测和比较。结果:不同方法制备的大鼠坐骨神经AECM在基底膜管完整性、细胞外基质(extracellular matrix,ECM)活性、去细胞程度和脱髓鞘程度方面无显著性差异。结论:应用优化的化学萃取脱细胞方法和低渗-酶消化法制备的AECM,均具有良好的仿生性,是修复周围神经缺损的适宜移植物。Objective：By comparing the composition of acellular extracellular matrix （AECM） obtained via different decellulariza- tion protocols, we aim at supplying experimental evidence for the solution of the source of grafts in clinical peripheral nerve repairs. Methods ：The transplants prepared through optimized chemical processing and with hypotonic - enzymatic method were obtained from rats. The construction and composition of AECM were evaluated by performing HE dyeing, chromotropic acid 2R - brilliant green stai- ning, immunohistochemistry and SDS - PAGE. Results：There was no significant difference in the integrity of vessel of basilar mem- brane, the activity of extracellular matrix （ ECM ） and the extent of removal of cells and myelin between different decellularization proto- cols. Conclusion： AECM obtained via optimized chemical processing and hypotonic - enzymatic method in rats appeared to be bionic, thus both scaffolds could be used as suitable transplants for effective repair of peripheral nerves.

关 键 词：脱细胞神经 化学 酶 萃取 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]