流产布鲁氏菌疫苗A19-ΔVirB12突变株生物学特性研究  

Biological characteristics of vaccine candidate A19-ΔVirB12 mutant strain of Brucella abortus

作　　者：易新萍[1] 谷文喜[1] 吴冬玲 李延涛 马晓菁[1] 叶锋[1] 刘丽娅[1] 薛晶[1] 钟旗[1] 

机构地区：[1]新疆维吾尔自治区畜牧科学院兽医研究所,乌鲁木齐830000 [2]新疆天康畜牧生物技术股份有限公司,乌鲁木齐830032

出　　处：《中国人兽共患病学报》2013年第9期836-840,共5页Chinese Journal of Zoonoses

基　　金：农业部公益性行业专项(201103008);国家科技支撑项目(2012BAD13B03);新疆维吾尔自治区高技术研究发展计划(200911109)联合资助~~

摘　　要：目的为了开发布鲁氏菌病新型标记疫苗,对流产布鲁氏菌A19-ΔVirB12突变株生物学特性研究。方法以亲本A19菌株为参照,对A19-ΔVirB12突变株的菌落形态、生物学特性、毒力、遗传稳定性、免疫原性进行实验比较。结果 A19-ΔVirB12突变株形态及生化特性同亲本株A19基本一致,其毒力弱于A19。A19-ΔVirB12突变株遗传稳定性良好且与A19具有相似的免疫原性。结论流产布鲁氏菌A19-ΔVirB12株毒力较低、遗传性状稳定、并具有良好的免疫保护效力,可作为新型动物布鲁氏菌病标记疫苗研制的候选株。The aim of this study is to determine the biological characteristics and immunogenicity of the candidate vaccine of A19-ΔVirB12 mutant strain for development of a novel marker brucellosis vaccine. Growth characteristics, staining proper ties, generation stability, virulence, and immunological protection between A19-ΔVirB12 and its parent strain A19 were com- pared. The results showed that the growth and biochemistry characteristics of A19-ΔVirB12 were similar to the parent strain. In the mice model, A19-ΔVirB12 strain was attenuated and induced the similar immunological protection levels as A19 vaccine strain. Also, it had good heredity stability. The results suggest that A19-ΔVirB12 mutant strain has low virulence, high genet ic stability, and good immunogenieity, and could be a new marker vaccines using for the prevention and control of animal brucellosis.

关 键 词：流产布鲁氏菌 A19-ΔVirB12突变株 稳定性 毒力 免疫原性 

分 类 号：S855.1[农业科学—临床兽医学]