伽马射线体外照射对细粒棘球蚴原头节的杀灭作用  

Killing effect on in vitro exposure of Echinococcus protoscoleces to Gamma-ray

作　　者：袁庆[1] 李波[2] 贺凯[2] 张孟瑜[2] 多吉[1] 陈川[2] 李兴[2] 向证文[2] 

机构地区：[1]四川省甘孜州人民医院,康定626000 [2]泸州医学院附属医院,泸州646000

出　　处：《中国人兽共患病学报》2013年第9期904-908,共5页Chinese Journal of Zoonoses

摘　　要：目的探讨细粒棘球蚴原头节体外照射γ-射线后导致的死亡、细胞内caspase-3的表达和显微结构的变化。方法在体外培养的基础上,用不同剂量的γ-射线对细粒棘球蚴原头节进行照射。对照射后各组原头节细胞内的caspase-3蛋白酶活性进行测定,观察期原头节死亡率,并在倒置显微镜下动态观察原头节形态结构的变化。结果原头节经过γ-射线体外照射后会出现死亡率升高,caspase-3表达增强现象,同时原头节形态结构出现肿胀、塌陷、碎裂的阶段性变化。结论γ-射线体外照射可以直接杀灭原头节,且诱导原头节细胞发生凋亡。In this study, we explore the killing effect, the expression of caspase-3 in the cells, and the changes in the mi croscopic structure of Echinococcus protoscoleces after extracorporeal Gamma-ray irradiation. The Echinococcus protoscoleces were treated with different doses of Gamma-ray irradiation. On the basis of in vitro culture, the protease activity of caspase-3 within various groups of protoscoleces ceils were tested after the irradiation, the death incidence of protoscoleces were ob- served, and dynamic observation were conducted for the changes in the morphology and structure of protoscoleces under invert ed microscope. It was found that after gamma-ray irradiation, the death incidence of protoscoleces were increased, and the ex pression of caspase-3 was enhanced~ meanwhile, phasal changes of swelling, collapse and breaking up appeared in the morpho- sis and structure of protoscoleces. The results indicate that extracorporeal Gamma-ray irradiation could directly disinfect proto scoleces and induce the apoptosis of protoscoleces cells.

关 键 词：细粒棘球蚴 原头节 γ-射线 杀灭 细胞凋亡 

分 类 号：R532.32[医药卫生—内科学]