抵当汤对高脂饮食胰岛素抵抗大鼠的瘦素、脂联素及其受体mRNA表达的影响  

Effects of Didangtang on the expression of adiponectin and leptin and their receptors mRNA in rat model with insulin-resistant induced by high fat diet

作　　者：丁宁[1] 张玲[1] 陈凤静[1] 刘国旗 张艳慧[1] 

机构地区：[1]河北医科大学,石家庄050091 [2]洛阳第一人民医院,洛阳471000

出　　处：《中药药理与临床》2013年第4期1-5,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：河北省科技支撑计划项目(10276105D-92)

摘　　要：目的:研究抵当汤对高脂饮食胰岛素抵抗大鼠的瘦素(LP)、脂联素(APN)及其受体mRNA表达的影响。方法:大鼠35只随机分为5组,空白对照组每日给予普通饲料和蒸馏水(10ml/kg)灌胃;模型组每日给予高脂饲料和蒸馏水(10ml/kg)灌胃;抵当汤低剂量组每日给予高脂饲料,低剂量抵当汤药液灌胃(1g/kg);抵当汤高剂量组每日给予高脂饲料,高剂量抵当汤药液灌胃(2g/kg);血脂康组每日给予高脂饲料,血脂康灌胃(0.2g/kg)。连续喂养8周,留取血清、血浆标本,进行生化指标及血清脂联素及血清瘦素的检测;迅速切取部分肝脏组织,进行脂联素及瘦素受体表达的检测。结果:模型组与空白组相比,大鼠脂联素均明显降低(P<0.01),瘦素增多(P<0.05);肝脏脂联素受体AR2mRNA)表达降低(P<0.05);瘦素受体OB-RmRNA表达明显降低(P<0.01);抵当汤低、高剂量组与模型组相比,大鼠APN增多(P<0.01),抵当汤低、高剂量组LP降低(P<0.05);抵当汤高剂量组大鼠AR2mRNA表达显著增多(P<0.01),抵当汤低剂量组AR2mRNA表达增多(P<0.05);抵当汤高剂量组大鼠OB-R mRNA表达明显增多(P<0.01)。结论:抵当汤可提高胰岛素抵抗大鼠胰岛素敏感性,防治胰岛素抵抗;抵当汤可升高胰岛素抵抗大鼠血清脂联素水平,降低血清瘦素水平;抵当汤可上调胰岛素抵抗大鼠肝脏脂联素受体AR2mRNA、瘦素受体OB-RmRNA的表达。Objective： To study the effects of Didangtang on the expression of adiponectin and leptin and their receptors mRNA in rat model with insulin-resistant induced by high fat diet.Methods： 35 health and clean level Wistar male rats were chosen and randomly divided into 5 groups： control group,model group,low-dose Didangtang group,high-dose Didangtang group and Xuezhikang group with 7 rats each.The rats of control group were fed with normal diet and distilled water（10ml / kg） daily,model group was fed high fat diet and distilled water（10ml/kg） daily,low-dose Didangtang group was fed high fat diet and Didingtang（1g/kg） daily,high-dose Didangtang group was fed high fat diet and Didingtang（2g / kg） daily,Xuezhikang group was fed high fat diet and Xuezhikang（0.2g / kg） daily.After 8 weeks of feeding,take blood by decapitate.Blood was taken to measure biochemical markers and serum adiponectin and leptin.The liver tissue was taken promptly to measure AR2mRNA,OB-R mRNA.Results： Didangtang can reduce the IR rats serum leptin levels,raise serum adiponectin levels and increase the expression of adiponectin receptor and leptin receptor in the liver tissue of IR rats.Conclusion： Didangtang can improve insulin sensitivity.Improve insulin resistance.Didangtang is an ideal medicine in preventing and treating dyslipidemia.

关 键 词：抵当汤 脂联素 瘦素 脂联素受体 瘦素受体 胰岛素抵抗 

分 类 号：R285[医药卫生—中药学]