桂枝挥发油与桂皮醛对病毒性肺炎小鼠死亡保护作用及TLR/IFN信号机制研究  

The effects of the volatile oil of Ramulus Cinnamomi and cinnamaldehyde on death protection rate in H1N1 -infected mice and mechanism of TLR/IFN signal pathway

作　　者：刘蓉[1] 苟玲[1] 于柳[1] 何婷[1] 杨靖[1] 王哲[1] 龚锡平[1] 汤奇[1] 刘金伟[1] 曾南[1] 

机构地区：[1]成都中医药大学药学院中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137

出　　处：《中药药理与临床》2013年第4期33-36,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金项目(30973923)(8173637);"2010年度高层次留学人才回国资助人选"项目、"2010年度留学回国人员科技活动项目择优资助"项目(国家人社部人社厅函[2010]411、412号);四川省教育厅培育项目(12ZB037)

摘　　要：目的:观察桂枝挥发油、桂皮醛的抗甲型流感病毒A/PR/8/34(H1N1)作用,探讨其TLR/IFN信号通路机制。方法:制备流感病毒性肺炎小鼠模型,观察药物对模型小鼠的死亡保护作用;ELISA法测定模型小鼠血清IFN-α、IFN-β水平,Real-Time PCR法测定模型小鼠肺组织TLR7、MyD88、TRAF-6 mRNA的表达量。结果:桂枝挥发油0.348 mg/kg,桂皮醛0.264 mg/kg、0.132mg/kg治疗给药,及桂枝挥发油0.348 mg/kg预防给药,均能明显提高模型小鼠的死亡保护率。桂枝挥发油0.348 mg/kg显著提高模型小鼠血清IFN-α含量及肺组织TLR7 mRNA表达水平,桂皮醛0.264 mg/kg显著升高IFN-β含量。结论:桂枝挥发油、桂皮醛能提高流感病毒性肺炎小鼠的死亡保护率,表现出抗流感病毒作用,抗病毒作用机制与激活TLR7信号通路,促进IFN的分泌有关。Objective： To study the anti-influenza A virus A /PR /8 /34（H1N1） effect of volatile oil of Ramulus Cinnamomi（VORC） and cinnamaldehyde in vivo,and reveal its mechanism of TLR / IFN signaling pathway.Methods： Mice were infected with H1N1 virus,and the death protection rate of VORC and cinnamaldehyde were observed.The serum levels of IFN-α,IFN-β in H1N1-infected mice were measured by ELISA.Real-time RT-PCR was used to detect the expressions of TLR7,MyD88 and TRAF-6 mRNA in lung tissue.Results： VORC（0.348 mg / kg） and cinnamaldehyde（0.264,0.132 mg / kg） significantly increased the death protection rate in therapeutic administration way,while VORC（0.348 mg / kg） significantly increased the death protection rate in preventive administration way.VORC（0.348 mg / kg） and cinnamaldehyde（0.264 mg / kg） obviously increased the serum levels of IFN-α and IFN-β separately.VORC（0.348 mg / kg） obviously increased the expression of TLR7 mRNA.Conclusion： VORC and cinnamaldehyde could significantly increase the death protection rate in H1N1-infected mice by therapeutic way.The anti-influenza A virus mechanism was related to activating TLR7 signaling pathway and regulating the levels of IFN-α,IFN-β.

关 键 词：桂枝挥发油 桂皮醛 甲型流感病毒A PR 8 34(H1N1) TLR IFN信号 

分 类 号：R285[医药卫生—中药学]