大黄素对大鼠肝纤维化的影响及机理研究  

Effects of emodin on hepatic fibrosis in rats and underlying mechanisms

作　　者：王晓丽[1] 张英博[2] 李雪岩[2] 牛英才[1] 綦艳秋[3] 朱兰芹[3] 董妙先[2] 

机构地区：[1]齐齐哈尔医学院医药科学研究所,齐齐哈尔161006 [2]齐齐哈尔医学院中心实验室,齐齐哈尔161006 [3]齐齐哈尔医学院卫生所,齐齐哈尔161006

出　　处：《中药药理与临床》2013年第4期56-58,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金项目(81073080);黑龙江省教育厅科学技术研究面上项目(11551551)

摘　　要：目的:观察大黄素对抗CCl4诱导大鼠肝纤维化的作用及对肝脏组织α-SMA和Smad7蛋白表达的影响。方法:SD大鼠50只随机分为空白对照组(n=10)、CCl4组(n=20)和大黄素+CCl4组(n=20),共3组。用CCl4皮下注射制备大鼠肝纤维化模型。大黄素干预12周后处死大鼠。采用Masson染色法观察肝脏组织的胶原纤维改变,Western blot法检测α-SMA和Smad7蛋白表达水平。结果:CCl4用量为2 ml/kg体重,每周2次,共12周。大黄素按20 mg/kg灌胃处理,每天1次,连续12周。Masson染色结果显示,与空白组相比,CCl4组大鼠肝组织胶原纤维显著增加;与CCl4组比较,大黄素+CCl4组胶原纤维显著减少。Western blot结果显示,与空白组相比,CCl4组α-SMA蛋白表达显著上调,而Smad7蛋白表达显著下调;与CCl4组相比,大黄素+CCl4组α-SMA蛋白表达显著下调,而Smad7蛋白表达显著上调。结论:大黄素具有逆转CCl4诱导大鼠肝纤维化的作用。其机制可能通过上调Smad7、下调α-SMA蛋白表达,进而抑制肝星状细胞活化有关。Objective： To investigate the inhibitory effect and possible mechanism of emodin on hepatic fibrosis induced by carbon tetrachloride（CCl 4）.Method： Studies were conducted in 50 male Sprague-Dawley rats,and they were randomly divided into 3 groups,named as blank controls group（n = 10）,CCl 4 group（n = 20）,and emodin ＋ CCl 4 group（n = 20）.The rat models of experimental hepatic fibrosis were established by injection with CCl 4.The rats treated with CCl 4 received emodin via oral administration at a dosage of 20 mg·kg·d at the same time.Rats were injected olive oil served as control groups.After 12 weeks,the liver histopathology was examined by Masson trichrome staining.Smad7 and α-SMA protein expression in hepatic tissue were detected by Western blot.Result： Compared with the normal group,the fibrotic area was increased in CCl 4 groups（P ＆lt; 0.05）.The fibrotic area in emodin ＋ CCl 4 group was decreased compared with the CCl 4 group（P ＆lt; 0.05）.Compared with the blank controls group,the α-SMA protein expression was up-regulated in CCl 4 groups（P ＆lt; 0.05）.The α-SMA protein expression in emodin ＋ CCl 4 group was down-regulated compared with the CCl 4 group（P ＆lt; 0.05）.The protein expression levels of Smad7 in the CCl 4 group was significantly down-regulated than that in the normal group,and that from CCl 4 ＋ emodin group was significantly up-regulated than in the CCl 4 group（P ＆lt; 0.05）.Conclusion： emodin exert anti-hepatic fibrosis effect in rats via inhibition of HSC activation by up-regulation expression of Smad7 and down-regulated expression of α-SMA in liver tissue.

关 键 词：大黄素 肝纤维化 Α-SMA SMAD7 MASSON染色 

分 类 号：R285[医药卫生—中药学]