葡萄籽原花青素对肾血管性高血压大鼠血压和TNF-α水平的影响  

Effects of grape seed procyanidin on the blood pressure and TNF-α levels in renovascular hypertensive rats

作　　者：张先杰[1] 郭荣年[1] 张妍[1] 孙柳青[1] 唐剑涛[2] 靳俊峰[1] 吴秀香[1] 

机构地区：[1]遵义医学院珠海校区病理学与病理生理学教研室,珠海519090 [2]遵义医学院珠海校区计算机教研室,珠海519090

出　　处：《中药药理与临床》2013年第4期70-73,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：贵州省科技厅资助项目(黔科合J字[2008]2317);贵州省高层次人才科研条件特助经费资助项目(TZJF-2008年58号);贵州省科技计划项目(黔科合SY字(2012)3114);贵州省优秀科技教育人才省长专项资金项目(黔省专合字(2012)91)

摘　　要：目的:探讨葡萄籽原花青素(GSP)对肾血管性高血压(RH)大鼠动态血压和血清中肿瘤坏死因子-α(TNF-α)含量、腹主动脉、肾皮质和海马组织中TNF-α蛋白表达的影响。方法:采用两肾一夹(2K1C)法建立RH大鼠模型,并设立假手术组(n=7)。术后2周,选取鼠尾动脉收缩压(SBP)超过130mmHg的大鼠28只为RH大鼠,并随机分为4组(n=7):高血压模型组;低剂量葡萄籽原花青素治疗组(50mg/kg/d);高剂量葡萄籽原花青素治疗组(200mg/kg/d)及卡托普利阳性对照治疗组(30mg/kg/d),观察各组大鼠尾动脉SBP的动态变化,治疗6周后,分别采用ELISA和Western blotting法检测各组大鼠血清和腹主动脉、肾皮质及海马组织中TNF-α水平。结果:应用葡萄籽原花青素治疗后,与假手术组相比,高血压模型组大鼠尾动脉SBP一直明显升高;治疗6周后,高血压模型组大鼠血清中TNF-α含量和腹主动脉、肾皮质及海马组织中TNF-α的蛋白表达水平均较假手术组明显升高。与高血压模型组相比,用葡萄籽原花青素治疗2周即可使RH大鼠尾动脉SBP降低,用药6周后降压效果更明显。同时亦能显著降低RH大鼠血清中TNF-α含量和腹主动脉、肾皮质及海马组织中TNF-α的蛋白表达水平,其中以高剂量葡萄籽原花青素治疗组的作用尤为明显,与卡托普利治疗组的作用相当。结论:葡萄籽原花青素可以通过减少RH大鼠体内促炎介质TNF-α的生成而发挥降压和抗炎作用。Objective： To explore the effects of grape seed procyanidins（GSP） on the dynamic blood pressure and the level of tumor necrosis factor-α（TNF-α） in serum,the expressions of TNF-α protein in abdominal aorta,renal cortex and hippocampus in renovascular hypertensive（RH） rats.Methods： The RH rat model was established by two-kidney one clip（2K1C） method,7 rat with sham operation were served as controls.Two weeks after operation,28 rats were selected according to the increased tail systolic pressure above 130mmHg and randomly divided into 4 groups（n = 7） ： RH model group,low GSP-treated group（50 mg / kg）,high GSP-treated group（200 mg / kg） and captopriltreated group（30 mg / kg）.The dynamic tail systolic pressure was observed,ELISA and Western blotting were performed to determine the TNF-α level in serum and the expressions of TNF-α protein in abdominal aorta,renal cortex and hippocampus in each group.Results： Compared with control group,the tail systolic pressure was increased significantly during the GSP treatment.6 weeks after treatment,the TNF-α level in serum and the expressions of TNF-α protein in abdominal aorta,renal cortex and hippocampus in RH rats were increased markedly than that of control group.Compared with RH model group,treatment with GSP for 2 weeks could decrease the tail systolic pressure of RH rats.This effect was more notable after 6 weeks treatment.Meanwhile,GSP could reduce the TNF-α level in serum and the expressions of TNF-α protein in abdominal aorta,renal cortex and hippocampus of RH rats significantly.These effects were more notable in high GSP-treated group,and equal to captopril-treated group.Conclusion： GSP treatment significantly decreases tail systolic pressure and attenuates the inflammation in RH rats by reducing the inhibiting inflammatory mediator TNF-α production.

关 键 词：葡萄籽原花青素 肾血管性高血压 TNF-Α 

分 类 号：R285[医药卫生—中药学]