Generation of RAG 1- and 2-deficient rabbits by embryo microinjection of TALENs  

作　　者：Jun Song Juan Zhong Xiaogang Guo Yongqiang Chen Qingjian zou Jiao Huang Xiaoping Li Quanjun Zhang Zhiwu Jiang Chengcheng Tang Huaqiang Yang Tao Liu Peng Li Duanqing Pei Liangxue Lai 

机构地区：[1]1Key Laboratory of Regenerative Biology, Chinese Academy of Sciences,Guangdong Provincial Key Laboratory of Stem Cells and RegenerativeMedicine, South China Institute for Stem Cell Biology and RegenerativeMedicine, Guangzhou Institutes of Biomedicine and Health, Guangzhou,Guangdong 510530, China,' [2]College of Veterinary Medicine, Jilin Univer-sity, Changchun, Jilin 130062, China, [3]School of Life Sciences, Universityof Science and Technology of China, Hefei, Anhui 230027, China

出　　处：《Cell Research》2013年第8期1059-1062,共4页细胞研究（英文版）

摘　　要：Dear Editor, The traditional approach for the generation of gene- targeted animals is homologous recombination （HR） conducted in embryonic stem （ES） cells followed by chimera technology, such as in mice and rats, or HR in somatic cells combined with nuclear transfer in those animals in which germline-competent ES cells are not available, such as pigs, sheep, goats, and cattle. How- ever, no germline-competent rabbit ES cells are yet available. Furthermore, the efficiency of rabbit cloning is so low that no gene-targeted rabbits have been produced thus far by somatic cell nuclear transfer aooroaches.

关 键 词：兔胚胎 显微注射 RAG 体细胞核移植 代数 缺陷 ES细胞 生殖能力 

分 类 号：Q78[生物学—分子生物学] S852.2[农业科学—基础兽医学]