电感耦合等离子体质谱法测定血清镁  

Determination of magnesium in serum by inductively coupled plasma mass spectrometry

作　　者：闫颖[1] 汪静[1] 赵海舰[1] 胡翠华[1] 马嵘[1] 张江涛[1] 陈文祥[1] 张传宝[1] 

机构地区：[1]卫生部北京医院卫生部临床检验中心,100730

出　　处：《中华检验医学杂志》2013年第8期738-742,共5页Chinese Journal of Laboratory Medicine

基　　金：国家高技术研究发展计划资助项目（2011AA02A102,2011AA02A116）;国家科技支撑计划资助项目（2012BA137801）

摘　　要：目的建立一种应用电感耦合等离子体质谱（ICP—MS）测定血清中镁（Mg）含量的候选参考方法。方法验证性研究。以铝（Al）作为Mg的内标，用重量法加入到标准溶液和血清中，样品经过硝酸消解、稀释，以ICP—MS测定其^27Mg/^27Al同位素比值，选择与样品相邻的一对标准溶液校准样品中Mg的浓度，采用包括法定量。对方法的线性范围、精密度、回收率以及正确度进行验证，并计算测量不确定度，参加国际比对。结果该方法的线性范围为0～0．5¨g／g（R。=0．99998），测定2份血清中Mg的分析回收率分别为100．22％和100．11％（n=3），测定3份血清的重复性CV分别为0．35％、0．36％和0．41％（n=9）。正确度验证显示测定标准参考物质SRM909b和SRM956b的结果均在认定值范围内，SRM909b两水平血清测定总变异系数分别为0．54％、0．53％，SRM956b三水平血清测定总变异系数分别为0．30％、0．50％和0．47％。参加国际参考实验室间比对结果理想。结论本研究建立了ICP—MS测定血清Mg的测量程序，具有良好的线性范围、精密度和回收率，且此法操作简单快速，有望成为血清Mg测定的候选参考方法。Objective To develop a candidate reference method for the determination of magnesium in serum by inductively coupled plasma mass spectrometry method （ICP-MS）. Methods Aluminum was selected as internal standard of magnesium and spiked gravimetrically into magnesium standard solutions and serum samples. Then the 24 Mg/27 Al isotope ratios of digested and diluted samples were obtained by ICP-MS, the magnesium concentrations in serum were calibrated basing on the bracketing method. Results The analytical recoveries for 2 samples were 100. 22% and 100. 11% respectively （n = 3）. The precision for 3 samples were 0. 35% , 0. 36% and 0. 41% respectively （ n = 9 ） . The results on Standard Reference Materials SRM909b and SRM956b gave excellent agreement with the certified values. The results of SRM909b gave the coefficients of variation （CV） of 0. 54% and 0. 53% for 2 samples, and the results of SRM956b gave 0. 30%, 0. 50% and 0. 47% for 3 samples. The method has been successfully applied to the RELA inter-laboratories comparison. Conclusions The ICP-MS method is proved to be not only precise and accurate, but also quick and convenient for measuring magnesium in serum. It is promising to be a candidate reference method.

关 键 词：镁 质谱分析法 分光光度法 原子 

分 类 号：R446.1[医药卫生—诊断学]