辛硫磷亚致死剂量对桑粉虱解毒酶活性的影响  

Effects of sublethal doses of phoxim on enzyme activity of Pealius mori(Takahashi)

作　　者：柴建萍[1] 倪婧[1] 江秀均[1] 罗雁婕[1] 谢道燕[1] 达爱斯[1] 黄平[1] 

机构地区：[1]云南省农业科学院蚕桑蜜蜂研究所,云南蒙自661101

出　　处：《南方农业学报》2013年第7期1115-1119,共5页Journal of Southern Agriculture

基　　金：国家自然科学青年基金项目(NSFC31201546);现代农业产业技术体系建设专项资金项目(CARS-22-SYZ27);云南省自然科学基金项目(2010ZC170)

摘　　要：【目的】研究辛硫磷对桑粉虱成虫的毒力及其亚致死剂量作用下桑粉虱成虫谷胱甘肽S-转移酶(GST)、羧酸酯酶(CarE)、乙酰胆碱酯酶(AchE)的活性变化,为桑粉虱抗药性研究及杀虫剂合理使用提供依据。【方法】采用闪烁管药膜法测定辛硫磷对桑粉虱亚致死剂量LC10、LC30、LC50,玻璃均浆器冰浴提取经过处理的桑粉虱酶液,用BIO-RAD全波长微孔板酶标读数仪测定GST、CarE、AchE。【结果】辛硫磷对桑粉虱成虫亚致死剂量LC10、LC30、LC50分别为0.0090、0.0899、0.4427μg/mL,随着辛硫磷亚致死剂量浓度增大,桑粉虱成虫GST活性显著增大、CarE及AchE活性显著下降。【结论】GST在桑粉虱对辛硫磷的解毒代谢中发挥作用。[Obejective]The virulence and effects of sublethal dose of phoixm on activities of glutathione s-tranferase,car- boxylesterase and acetylcholinesterase from pest insect Pealios mori （Takahashi） were studied to provide references for P. mori （Takahashi） resistance research and suitable application of pesticide. [Method]The sublethal doses of phoxim LCl0, LC30, LC50 were measured by glass tube residual film. The enzyme solution of P. moll （Takahashi） was extracted by glass BioMasher. The enzyme activities of glutathione s-tranferase, earboxylesterase and aeetylcholinesterase of P. mori （Taka- hashi） was detected by BIO-RAD Multiskan Spectrum. [Result ]The enzyme activity of glutathione s-tranferase increased no- tably as the sublethal doses of phoxim disoposal concentration went up, while the enzyme activities of carboxylesterase and aeetyleholinesterase obviously decreased. [Conclusion]The glutathione s-tranferase played a role in the course of phoixm detoxifcation in P. mori （Takahashi）.

关 键 词：桑粉虱 亚致死剂量 谷胱甘肽S-转移酶 羧酸酯酶 乙酰胆碱酯酶 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治] Q965.9[农业科学—植物保护]