南海稀有放线菌的分离及其卤化酶基因分析  

The separation of rare actinomycetes from South China Sea and analysis of its halogenase gene

作　　者：陈名洪[1] 王海龙[1] 林如[1] 谢阳[1] 连云阳[1] 江红[1] 

机构地区：[1]福建省微生物研究所福建省新药(微生物)筛选重点实验室,福州350007

出　　处：《中国抗生素杂志》2013年第9期658-663,共6页Chinese Journal of Antibiotics

基　　金：福建省自然科学基金项目(2012J01093);福建省科技重点项目(2011R1010-2;2012Y0077);国家科技重大专项(2012ZX09301002-003)

摘　　要：目的探究中国南海沉积物中放线菌的多样性并从中发现合成药物先导化合物的新菌源。方法采用6种选择性培养基分离24份来自南海沉积物样品中含有的放线菌。挑选不同培养特征的放线菌进行初步分类鉴别、16S rRNA基因序列系统进化分析及基于PCR的卤化酶基因筛选。结果共分离到900株放线菌,挑选的210株放线菌分别属于放线菌9个科,13个属,210株菌中有38株含有卤化酶的生物合成基因片段。结论海洋环境蕴含丰富的放线菌资源并具有产生天然的有活性卤化物的潜能。Objective To investigate the diversity of actinomycetes isolated from South China Sea marine sediments and isolate new actinomycetes for discovering compounds of pharmaceutical importance. Methods Six selective media were used to isolate actinomycetes from 24 marine sediments of South China Sea. Actinobacterial diversity in these sediments was investigated by phylogenetic analysis based on 16S rRNA gene sequences. To detect potential producer strains of halometabolites, PCR based screening strategy was used. Results A total of 900 strains of actinomycetes were isolated and 210 representative isolates were selected on the basis of their morphologies on different media. 16S rRNA gene sequences phylogenetic analysis showed that these strains belonged to nine families including thirteen genera. 18% of the above 210 strains were detected and found containing halogenases gene. Conclusion The results confirm that marine sediments are rich source of rare actinomycetes and the actinomycetes from marine environment have the potential of producing natural active halometabolites.

关 键 词：南海 稀有放线菌 多样性 卤化酶 

分 类 号：R978.1[医药卫生—药品]