两性霉素B与鞘磷脂单分子层的相互作用  

Interaction of Amphotericin B/Sphingomyelin Monolayers at the Air/Water Interface

作　　者：乔进京[1] 孙润广[1] 郝长春[1] 杨静[1] 马秀梓[1] 王小梅[1] 

机构地区：[1]陕西师范大学物理学与信息技术学院,生物物理与生物医学工程研究室,西安710062

出　　处：《高等学校化学学报》2013年第9期2125-2130,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20772077);教育部高等学校博士学科点专项科研基金(批准号:200807180001);陕西省自然科学基础研究计划(批准号:2012JQ1002);中央高校基本科研业务费专项项目(批准号:GK201102029)资助

摘　　要：选取哺乳动物生物膜中的重要脂质分子鞘磷脂(SM)作为单分子膜的基本组分,采用Langmuir-Blodgett(LB)膜技术研究了不同比例的两性霉素B/鞘磷脂单层膜的表面压力-平均分子面积(π-A)曲线以及基于π-A曲线的混合性分析,同时通过原子力显微镜(AFM)研究了其表面形态的变化.结果表明,组分间的摩尔比和表面压力对混合单层膜稳定性、混合性以及分子间相互作用具有重要影响.In this work, we chose sphingomyelin （SM） as the basic components of the monolayer, which is widespreaded in mammalian cells and takes an important role in biochemical reactions. Different proportion of amphotericin B/sphingomyelin monolayer＇ s surface pressure and mean molecular area（7r-A） curve was inves- tigated by the Langmuir-Blodgett （LB） films technology, thermodynamic characteristics were also analyzed based on the 7r-A curve and the morphological changes of the surface were observed by atomic force microscopy （AFM）. The results showed that the compressibility, stability and thermodynamic characteristics of AmB/SM monolayer depended on the mole faction of AmB and the surface pressure obviously. This may be related to the inhibition of Amphotericin B.

关 键 词：两性霉素B 单层膜 鞘磷脂 π-A曲线 原子力显微镜 

分 类 号：O621[理学—有机化学] O641[理学—化学]