黑穗醋栗愈伤组织诱导研究  

Study on the Callus Induction of Blackcurrant

作　　者：李金英[1] 赵春莉[1] 张馥茜[1] 张志东[1] 程国栋[1] 

机构地区：[1]吉林农业大学园艺学院,吉林长春130118

出　　处：《北方园艺》2013年第17期87-90,共4页Northern Horticulture

基　　金：吉林省教育厅科研资助项目(吉教科合字[2011]第45号)

摘　　要：以黑穗醋栗品种"寒丰"和"早生黑"的无菌组培苗为试材,选取叶片、叶柄及茎段为外植体,研究了不同外植体对愈伤组织诱导率的影响,以及不同植物生长调节剂及配比对愈伤组织诱导、继代及植株再生的影响。结果表明:叶柄为愈伤组织诱导最适宜的外植体;2,4-D在愈伤组织诱导可再生过程中起了明显的积极作用,适宜浓度为1.0mg/L。黑穗醋栗叶片、叶柄和茎段不同程度的诱导出了愈伤组织;幼嫩的无芽茎段在适宜的培养基中通过愈伤组织途径,初步分化出了不定芽,为黑穗醋栗愈伤组织再生和遗传转化的研究奠定了一定基础。Taking the plantlet of two varieties of blackcurrant ＇ Hanfeng＇ and ＇ Fortodi＇ as materials, with the leaves, the petioles and the stems as explants, the effect of explants on callus induction rate was studied, and the influence of different hormones and concentrations on callus induction,callus subculture and plant regeneration were investigated. The results showed that the petioles was the best explants for callus induction. The different concentration of 2,4-D that was used in callus growing had obvious effect on the callus differentiation, and the optimal concentration of 2,4-D was 1.0 mg/L. Callus could be induced by culturing leaves, petioles and stems of blackcurrant in vitro. The young stems could preliminary differentiate the adventitious buds in a suitable medium through callus. It laid a foundation for the blackcurrant callus regeneration and genetic transformatiorL

关 键 词：黑穗醋栗 ‘寒丰’ ‘早生黑’ 愈伤组织 诱导 

分 类 号：S663.9[农业科学—果树学]