降低栒子试管苗培养成本的方法研究  

Study on Reducing Cost of Cotoneaster hjelmqvistii Plantlet in vitro

作　　者：梁发辉[1] 柴慈江[1] 何丹[1] 史燕山[1] 骆建霞[1] 卢兴霞[1] 

机构地区：[1]天津农学院园艺系,天津300384

出　　处：《北方园艺》2013年第17期107-109,共3页Northern Horticulture

基　　金：国家科技部星火计划资助项目(2008GA610015);天津市科委资助项目(08ZHXHNC07000)

摘　　要：以栒子为试材,分别用绵白糖代替蔗糖、用自来水代替蒸馏水进行栒子茎芽增殖培养,探讨降低栒子试管苗成本的方法。结果表明:各处理间栒子试管苗的增殖系数没有显著差异;用绵白糖代替蔗糖进行栒子试管苗的生根培养,各处理间在根长、根数、发根率上无显著差异。将栒子试管苗放置在不同培养环境进行生根培养发现,温室条件下栒子试管苗生根效果要优于培养室环境。可见,在栒子试管苗增殖培养中可以用绵白糖代替蔗糖、用自来水代替蒸馏水,生根培养中也可用绵白糖替代蔗糖;栒子试管苗的生根培养可以在一定时期转入日光温室中进行。上述措施可以明显降低栒子试管苗培养成本。Taking Cotoneaster hjelmqvistii as material, the soft white sugar and tap water were respectively used to instead of sucrose and distilled water in micro-shoot proliferation culture to reduce the cost of Cotoneaster hjelrnqvistii plantlet in vitro. The results showed that the micro-shoots multiplication had no significant difference among the treatments; during rooting culture with soft white sugar instead of sucrose, there was no significant difference among all treatments. Cotoneaster plantlets were placed in different culture environments for rooting, and under greenhouse the rooting effect was better than that under the training room environment. In conclusion,the soft white sugar and the tap water can be used to replace sucrose and distilled water for the Cotoneaster hjelmqvistii plantlets proliferation culture, also the soft white sugar can be used to replace sugar in the plantlet rooting. The plantlet rooting can be carried out under the greenhouse conditions. These methods can significantly reduce the cost of Cotoneaster hjelmqvistii plantlet in vitro.

关 键 词：栒子 试管苗 茎芽增殖 生根培养 成本 

分 类 号：Q813[生物学—生物工程]