聚乙烯亚胺介导DNA转染肺癌A549/DDP细胞的研究  

Study on polyethlenimine-mediated DNA transfer into A549/DDP lung cancer cells

作　　者：李怀永[1] 邵淑丽[1] 张伟伟[1] 李铁[1] 李玲玲[1] 夏艳[1] 张希锐[1] 贾晓龙[1] 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006

出　　处：《黑龙江畜牧兽医》2013年第9期22-24,178,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：黑龙江省自然科学基金项目(C200624;C201241);黑龙江省教育厅科学技术项目(11511447;12511611)

摘　　要：为了确定聚乙烯亚胺(polyethlenimine,PEI)转染肺癌A549/DDP细胞的最佳条件,试验以PEI为载体,将含有绿色荧光蛋白的pRNAT-H1.1/Shuttle-GFP表达载体导入肺癌A549/DDP细胞中,在倒置荧光显微镜下观察不同PEI和DNA质量比、细胞密度、血清含量,以及是否去内毒素和线性化重组质粒对聚乙烯亚胺转染肺癌A549/DDP细胞的影响。结果表明:在质量比为2∶1、肺癌A549/DDP细胞于含10%血清中生长至密度为80%时,转染48 h的转染率最高。To investigate the best conditions of polyethylenimine - mediated DNA transfer into A549/DDP lung cancer cells, the expression vector pRNAT - HI. 1/Shuttle - GFP containing green fluorescent protein （GFP） was induced in A549/DDP lung cancer cells using polyethleni- mine （PEI） as a vector. The effects of different PEI/DNA ratios, cell confluence, content of serum, endotoxin and linearization on the transfec- tion efficiencies of PEI were observed using an inverted fluorescence microscope. The results showed that the transfection efficiency of PEI was the highest after 48 h of transfection, if it satisfied the following conditions ： PEI/DNA mass ratio was 2： 1, the density of A549/DDP was 10%, and the cell confluence in serum was 80%.

关 键 词：聚乙烯亚胺 转染 A549 DDP细胞 

分 类 号：Q813[生物学—生物工程]