新佐剂——Montanide ISA 50V2在马免疫中的应用研究  

Application of a new adjuvant-Montanide ISA 50V2 in horse immunization program

作　　者：刘卫涛[1] 柳洲[1] 李育合[1] 谢小荣[1] 孟祥玉[1] 张廷芬[1] 高建军[1] 

机构地区：[1]兰州生物制品研究所有限责任公司,兰州730046

出　　处：《微生物学免疫学进展》2013年第4期42-44,共3页Progress In Microbiology and Immunology

摘　　要：目的研究新佐剂-Montanide ISA 50V2在马免疫中的应用,制定合理的免疫程序,提高马免疫血清效价。方法用Montanide ISA 50V2乳化A型和F型肉毒类毒素制备成油乳抗原,免疫家兔和马,并与福氏不完全佐剂进行对比。结果在马免疫程序中,Montanide ISA 50V2与福氏不完全佐剂交替使用效果更好,免疫效价较高。结论在进一步试验确证后,将Montanide ISA 50V2作为佐剂应用于马免疫中,以获得更高的免疫血清的效价。Objective To improve the titers of antitoxin serum prepared in horses immunization program.Methods Montanide ISA 50V2 was used as a new adjuvant in immunization program for rabbits and horses.Montanide SA 50V2 and Clostridium botulinum type A and F toxoids antigens were emulsified to O / W emulsion,and then using this emulsion to immunize the horses,and compared with the conventional adjuvant（FIA） with the same antigen of clostridium botulinum type A and F toxoids.Results The immune effect was the best when two emulsions were alternately used,the titer of antitoxin serum was the highest.Conclusion Montanide ISA 50V2 could be used as a adjuvant in horses immunization program for production of higher titers of antitoxin serum.

关 键 词：Montanide ISA 50V2 福氏不完全佐剂 免疫程序 

分 类 号：R392[医药卫生—免疫学]