β-淀粉样蛋白和载脂蛋白E4降低神经元膜流动性  

Amyloid beta protein and apolipoprotein E4 decrease neuronal membrane fluidity

作　　者：田映红[1] 周丽华[1] 谢瑶[1] 姚志彬[1] 

机构地区：[1]中山医科大学解剖学教研室脑研究室,广东广州510089

出　　处：《中国神经科学杂志》2000年第3期210-214,共5页

基　　金：国家自然科学基金资助课题 !(3 96 70 2 44)

摘　　要：为了研究 Aβ和 Apo E对神经元膜流动性的影响 ,探索 Aβ和 Apo E的神经毒性机制 ,制备急性分离的新生 SD大鼠海马和皮质神经元细胞悬液 ,采用显微准弹性激光散射技术 ,分析单个神经元散射光强度的自相关函数 (ACF) ,通过公式由 ACF拟合出反映细胞膜流动性的频移线宽 Γ。结果发现 Aβ2 5～ 3 5和 Apo E4作用 5min和 3 5min后均降低海马和皮质神经元 ACF的衰减速率及频移线宽 Γ,而 Apo E3对两者无影响 ,提示 Aβ和 Apo E4均可降低神经元膜流动性。To explore the neurotoxic mechanism of Aβ and ApoE4 on neurons,freshly dissociated hippocampal and cortical neuron suspension of newborn SD rats were prepared. The neurons' autocorrelation function (ACF) of the scattering light intensity was analyzed by the microscope quasi elastic laser light scattering (MQLS) technique. The frequency shift line width Γ was fitted by ACF. The Γ can sympolize the cell membrane fluidity. The ACF attenuate rate and the frequency shift line width Γ of both hippocampal and cortical neurons were decreased by both Aβ and ApoE4 acting on neurons for 5 min and 35 min, while ApoE3 had no such effect. The data indicate that both Aβ and ApoE4 could decrease neuronal membrane fluidity,resulting in neurotoxicity.[

关 键 词：Β-淀粉样蛋白 载脂蛋白E 老年性痴果 膜流动性 

分 类 号：R341[医药卫生—基础医学]