十二指肠灌注亮氨酸对奶牛胰腺淀粉酶分泌的影响  

Duodenal Infusion of Leucine:Effects on Pancreatic Amylase Secretion of Dairy Cows

作　　者：刘烨[1] 刘凯[1] 徐明[1,2] 吕长荣[3] 曹阳春[1] 姚军虎[1] 

机构地区：[1]西北农林科技大学动物科技学院,杨凌712100 [2]内蒙古农业大学动物科学学院,呼和浩特010080 [3]西北农林科技大学动物医学学院,杨凌712100

出　　处：《动物营养学报》2013年第8期1785-1790,共6页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金(31001021);科技部国际科技合作与交流专项(2010DFB34230);国家科技支撑计划(2012BAD12B02;2012BAD39B05-2)

摘　　要：本试验旨在研究十二指肠灌注亮氨酸对奶牛胰腺淀粉酶分泌的影响。以4头周岁荷斯坦母牛[(215±7)kg]为试验动物,手术安装十二指肠胰液收集袋、灌注管和回流管以及颈静脉插管,采用4×4拉丁方试验设计,进行十二指肠亮氨酸灌注试验[(灌注水平分别为0、29.5、59.1和88.6μmol/(kg.h)]。结果表明,随亮氨酸灌注水平升高,血浆胰岛素和胆囊收缩素浓度升高(P<0.05),胰腺α-淀粉酶合成速率(U/h)呈先升高后降低趋势(P<0.01),在29.5μmol/(kg.h)灌注水平时最大。十二指肠灌注亮氨酸也极显著影响了胰腺α-淀粉酶分泌浓度(U/L,P<0.01;U/g prot,P<0.01)。结果表明,亮氨酸可能通过刺激胰岛素和胆囊收缩素释放,调控青年奶牛胰腺淀粉酶分泌功能,二者存在剂量效应。This experiment was conducted to evaluate the effects of duodenal leucine infusion on pancreatic a- amylase secretion of dairy cows. Four intravenously cannulated yearling Holstein cows [ （215 _ 7 ） kg ] with pancreatic pouch-duodenal reentrant cannulas, duodenal catheters and jugular catheters were used in a 4 x 4 Latin square with duodenal leucine infusions [ 0, 29.5, 59.1 and 88.6 /xmol/（ kg h） ]. The results showed as follows： plasma insulin and cholecystokinin concentrations were increased with leucine infusion level in- creasing （ P 〈 0.05 ）, and pancreatic oL-amylase production rate （U/h） was increased first and then decreased with leucine infusion level increasing （P 〈 0. 01 ）, in which the greatest value was observed at 29.5 p, mol/（ kg h）. Duodenal leucine infusion could also affect a-amylase concentration （ U/L, P 〈 0.01 ; U/g prot, P 〈0.01 ）. These data indicate that leucine can regulate pancreatic a-amylase secretion by stimula- ting the release of insulin and cholecystokinin of young daily cows, and there is a dose-effect relationship be- tween them. [ Chinese Journal of Animal Nutrition, 2013, 25 （ 8 ） ： 1785-1790 ]

关 键 词：亮氨酸 十二指肠灌注 淀粉酶 胰岛素 胆囊收缩素 

分 类 号：S828[农业科学—畜牧学]