甲醛对原癌基因c-myc、MDM2及抑癌基因p53的影响  

Expression of proto-oncogene c-myc,MDM2 and anti-oncogene p53 induced by different concentrations of formaldehyde

作　　者：李慧[1] 张玉超[1] 柯玉洁[1] 秦晓丹[1] 刘旭东[1] 问华肖[1] 熊棣[1] 李睿[1] 杨旭[1] 丁书茂[1] 

机构地区：[1]华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室,湖北武汉430079

出　　处：《中国环境科学》2013年第8期1483-1486,共4页China Environmental Science

基　　金：国家自然科学基金重点项目(51136002)

摘　　要：为研究吸入性甲醛的毒性能否进入动物骨髓组织,引起骨髓组织基因表达发生改变,选择小鼠某些原癌基因和抑癌基因为研究对象,以SPF级balb/c雄性小鼠为材料,采用动态吸入方式染毒2周(5+2模式),染毒浓度分别为0,0.5,3.0mg/m3,用半定量RT-PCR方法检测不同浓度甲醛对小鼠染毒后骨髓组织细胞中c-myc、MDM2和p53基因表达的变化.结果表明,在不同浓度的甲醛暴露条件下,与空白对照组相比,小鼠骨髓组织中的c-myc基因,MDM2基因和p53基因表达均发生改变,在3.0mg/m3浓度组与空白对照组存在显著差异(P<0.05),c-myc基因,MDM2基因呈现表达上调,p53基因则呈现表达下调.吸入性甲醛的毒性能进入动物骨髓组织,并能引起骨髓组织基因表达发生改变.In order to explore whether the inhaled formaldehyde have toxic effect on animal bone marrow tissue and induce changes in its gene expression profile, certain mice proto-oncogene and anti-oncogene were selected as the target in this study. SPF grade balb/c male mice were exposed to 0, 0.5and 3.0mg/m3 formaldehyde by inhaling, respectively, for two weeks in a simulated occupational exposure mode （2-day interval between each 5-day exposure）. After exposure, bone tissue was employed for the determination in the change of expression profile for c-my~, MDM2 and p53with semi-quantitative RT-PCR. Compared with the control group, the expression profile for e-rnyc, MDM2 and p53 all changed under different concentration of formaldehyde exposure. Especially in the mice group exposed to 3.0mg/m3 formaldehyde, there appears a significant difference （P〈0.05） in that MDM2 gene expression is up-regulated while p53gene expression is down-regulated. Inhaled formaldehyde is toxic to animal bone marrow tissue and will cause the change in its gene expression profile.

关 键 词：甲醛 SPF级Balb c纯系雄性小鼠 骨髓组织 c-myc MDM2 p53 

分 类 号：X503.22[环境科学与工程—环境工程]