甲醛对小鼠骨髓组织中GATA-2和C/EBPα表达的影响  

Expressions of GATA-2 and C/EBPα at mRNA and protein level in bone marrow of mice induced by formaldehyde exposure

作　　者：张玉超[1] 刘旭东[1] 李慧[1] 杨旭[1] 丁书茂[1] 

机构地区：[1]华中师范大学生命科学学院环境生物医学实验室遗传调控与整合生物学湖北省重点实验室,湖北武汉430079

出　　处：《环境与健康杂志》2013年第8期659-661,共3页Journal of Environment and Health

基　　金：国家自然科学基金重点项目(51136002);国家科技支撑计划重点项目(2012BAJ02B03-1)

摘　　要：目的探讨吸入式甲醛暴露对小鼠骨髓组织中转录因子GATA-2和CCAAT/增强子结合蛋白(C/EBPα)在mRNA以及蛋白表达水平的影响。方法将24只Balb/C小鼠随机分为3组,每组8只,采用仿真式口鼻暴露的方式,暴露于不同浓度的气态甲醛环境中(0、0.5和3.0 mg/m3),每天8 h,连续2周。染毒结束后,分别采用反转录PCR(RT-PCR)和酶联免疫吸附(ELISA)法测定小鼠骨髓组织中转录因子GATA-2和C/EBPαmRNA和蛋白的表达。结果与对照组相比,转录因子GATA-2和C/EBPαmRNA和蛋白的表达均随甲醛浓度的升高呈下降趋势,且甲醛浓度为3.0 mg/m3时,差异有统计学意义(P<0.05)。结论高浓度(3.0 mg/m3)甲醛暴露可以抑制小鼠骨髓组织中GATA-2和C/EBPαmRNA和蛋白的正常表达。Objective To investigate the impact of inhaled formaldehyde on the expression of transcription factor GATA-2 and C/EBPα at mRNA and protein level in mouse bone marrow. Methods A total of 24 Bale/C male mice were randomly divided into three groups, eight in each, and exposed to formaldehyde by nose-only inhalation at the doses of 0, 0.5, and 3.0 mg/m3 for 8 h/d, for two consecutive weeks. After two weeks of treatment, reverse transcription and polymerase chain reaction （RT-PCR） and enzyme-linked immunosorbent assay （ELISA） were used to detect the expressions of transcription factors GATA-2 and C/EBPα both at mRNA level and protein level. Results Compared with the control group, expression levels of GATA-2 and C/EBPα decreased significantly at the concentration of 3.0 mg/m3（p〈0.05）; And with the increase of formaldehyde concentration, the expression levels showed a downward trend. Conclusion Formaldehyde exposure may affect the expression levels of GATA-2 and C/EBPα and cause damage to hemopoietic function of bone marrow at high exposure level.

关 键 词：甲醛 骨髓 造血 转录因子 

分 类 号：R994.6[医药卫生—毒理学]