围生期暴露双酚A对青春期子代雌性大鼠糖代谢和血清炎症因子的影响  

Effects of perinatal exposure to bisphenol A on glucose metabolism and inflammatory factors in adolescent female offspring rats

作　　者：董幼丹[1] 张玲[1] 张洪远[1] 马翠翠[1] 贾丽红[1] 

机构地区：[1]中国医科大学公共卫生学院儿少卫生学教研室,辽宁沈阳110001

出　　处：《环境与健康杂志》2013年第8期665-667,共3页Journal of Environment and Health

基　　金：国家自然科学基金(81072311)

摘　　要：目的探讨围生期暴露双酚A(bispheno1 A,BPA)对青春期雌性子代大鼠体重、糖代谢及细胞炎症因子的影响。方法将21只清洁级SD妊娠大鼠分为3组,分别为对照(含1%无水乙醇的水)组和低(1μg/ml)、高(10μg/ml)浓度BPA暴露组,每组7只。从妊娠第6天至哺乳期(出生后20 d),母鼠采用自由饮水方式进行染毒;断乳后,雌性仔鼠采用自由饮水(不含BPA)方式继续喂养30 d。测定雌性仔鼠体重、空腹血糖、胰岛素水平及血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白介素6(interleukin-6,IL-6)水平,并计算胰岛素抵抗指数。结果各浓度BPA暴露组雌性仔鼠体重、空腹血糖、血清胰岛素水平和胰岛素抵抗指数、TNF-α、IL-6水平均明显高于对照组,差异有统计学意义(P<0.05,P<0.01)。结论围生期暴露BPA可引起子代雌性大鼠体重增高和胰岛素抵抗,并可诱发机体炎症反应。Objective To explore the effects of perinatal exposure to bisphenol A（BPA） on body weight and inflammatory factors in adolescent female offspring rats. Methods A total of 21 pregnant Sprague-Dawley rats were randomly divided into three groups, seven rats in each group, the control group （1% of anhydrous alcohol with water）,low dose （1 μg/ml） and high dose （10 μg/ml） groups of BPA exposed through drinking water,from gestation day 6 to the end of lactation respectively. Female offspring drank water without BPA for 30 days after weaning. Body weight,fasting serum glucose,insulin,necrosis factor-α （TNF-α） and interleukin-6 （IL-6） levels were determined, and insulin resistance index was calculated in female offspring after postnatal day 50. Results There were significantly higher body weight,serum glucose,insulin,and insulin resistance index,TNF-α and IL-6 levels in BPA-exposed female rats compared with the controls （P〈0.05 or P〈0.01）. Conclusion Perinatal exposure to BPA may result in body weight gain and insulin resistance,and induce inflammatory response in female offspring.

关 键 词：双酚A 肥胖 胰岛素抵抗 肿瘤坏死因子-α 白介素6 

分 类 号：R994.6[医药卫生—毒理学]