甲基对硫磷对小鼠黄体细胞的细胞间隙连接通讯和孕酮分泌的影响  

Effects of methyl parathion on gap junctional interc ellu lar communication and progesterone secretion of mouse luteal cells

作　　者：古威鹏[1] 夏莹莹[1] 晁璐[1] 潘楚洁[1] 吴继国[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院环境卫生学系,广东广州510515

出　　处：《环境与健康杂志》2013年第8期672-675,共4页Journal of Environment and Health

基　　金：高等学校博士学科点专项科研基金(20124433120021);广东省医学科学基金(A2011355);南方医科大学公共卫生与热带医学学院学生课外科研课题

摘　　要：目的探索甲基对硫磷（methylparathion，MP）对小鼠黄体细胞的细胞间隙连接通讯（gapiunctionalintercellularcommunication，GJIC）和孕酮分泌的影响。方法体外培养5周龄SPF级BALB／c小鼠黄体细胞，分别设MP暴露组（0、0．05、0．1、0．2、0．4、0．8、1．6mmol／L的MP处理3h）和G．1Ic促进剂——1μmol／L异丙肾上腺素预处理组及蛋白激酶A（PKA）专一抑制剂——10μmol／LH89预处理组与蛋白激酶C（PKC）专一抑制剂——10μmol／L恩扎妥林预处理组（预处理5min，0．8mmol／LMP处理3h）。采用ELISA法检测培养液中孕酮含量，划痕标记染料转移法测定贴壁细胞的GJIC。结果高浓度（0．2-1．6mmol／L）MP可明显抑制小鼠黄体细胞的GJIC和孕酮分泌（P〈0．05）；异丙肾上腺素、H89和恩扎妥林均能缓解MP对体外小鼠黄体细胞的GJIC和孕酮分泌的抑制作用（P〈0．05）。结论MP可通过抑制小鼠黄体细胞的GJIC导致孕酮分泌受抑制，而PKA与PKC参与该过程。Objective To study the effects of methyl parathion （MP） on progesterone secretion and gap junctional intercellular communication （GJIC） of mouse luteal cells. Methods Mouse luteal cells were treated by MP（0,0.05,0.1,0.2, 0.4,0.8 and 1.6 retool/L） for 3 h,isoprenaline （1 μmol/L,a promoter of GJIC） for 5 rain followed by treatment of 0.8 mmol/L MP for 3 h,H89 and enzastaurin （10 μmol/L,single or combined） for 5 min followed by treatment of 0.8 mmol/L MP for 3 h respectively. Progesterone content in medium was detected by ELISA kit specific for mouse progesterone and GJIC of mouse luteal cells was detected using the scrape loading and dye transfer technique. Results MP at high conncentrations （0.2-1.6 mmol/L） inhibited significantly progesterone secretion and GJIC of mouse luteal cells （P〈0.05）. Isoprenaline,H89 and enzastaurin could significantly alleviate the inhibition effect of MP on progesterone secretion and GJIC of mouse luteal cells （P〈0.05）. Conclusion MP may inhibit progesterone secretion of luteal cells through inhibition of GJIC,involving PKA and PKC in the process.

关 键 词：甲基对硫磷 细胞间隙连接通讯 生殖毒性 孕酮 

分 类 号：R994.6[医药卫生—毒理学]