HPLC-MS/MS联用技术测定人血浆中的比伐卢定  

Determination of Bivalirudin in Human Blood by HPLC-MS/MS

作　　者：李静[1] 刘文芳[1] 林阳[1] 吴伟[1] 赵桂平[1] 杨克旭[1] 所伟[1] 杜海燕[1] 仇琪[1] 

机构地区：[1]首都医科大学附属北京安贞医院,北京100029

出　　处：《中国药学杂志》2013年第16期1400-1403,共4页Chinese Pharmaceutical Journal

摘　　要：目的建立高效液相色谱-质谱联用法测定人血浆中比伐卢定浓度。方法选用AgilentSB-C18色谱柱，以甲醇-0.1％。甲酸水溶液为流动相，采用梯度洗脱进行分离，样品通过Oasis@HLB1cc萃取柱分离后进样，用3200Qtrap型质谱仪的多重反应监测（MRM）扫描方式进行检测。结果比伐卢定的线性范围为25-20000lag·L^-1，定量下限为25lag·L^-1。准确度与精密度结果显示方法日间、日内变异均小于6．63％，方法提取回收率大于80％，稳定性较好。结论该方法快速、灵敏、专属性强、重现性高，适用于比伐卢定浓度测定。OBJECTIVE To establish an HPLC-MS/MS method for the determination of bloodbivalirudin concentration. METHODS Following a SPE method,the analytes were separated on a C,8 column interfaced with a triple-quadrupole tandem mass spectrometer using positive electrospray ionization. RESULTS The calibration linear rang of bloodbivalirudin concentration was 25 -20 000 μg ＂ L^-l（r = 0. 996 9）. The between-day and within-day RSD% were less than 6. 63%. The recoveries were more than 80%. CONCLUSION The rapid, sensitive and reliable HPLC-MS/MS method can be applied to monitor blood- bivalirudin profile.

关 键 词：高效液相色谱-串联质谱法 比伐卢定 固相萃取法 

分 类 号：R917[医药卫生—药物分析学]