T-2毒素对大鼠卵巢颗粒细胞分泌激素的影响  

The Studies on the Effect and Mechanism of T-2 toxin on the Steroidogenesis of Rat Ovarian Grandlose in vitro

作　　者：丁慧昕[1] 董晓蓉[1] 文利新[1,2] 屠迪[1] 邬静[1] 袁莉芸[1] 

机构地区：[1]湖南农业大学动物医学院,湖南长沙410128 [2]长沙绿叶生物科技有限公司,湖南长沙410125

出　　处：《中国兽医杂志》2013年第7期30-31,共2页Chinese Journal of Veterinary Medicine

基　　金：湖南省教育厅一般项目(08C431);湖南农业大学动物保健工程中心绿叶基金项目;湖南农业大学青年基金(08LY01)

摘　　要：为了研究T-2毒素对卵巢颗粒细胞(GC)分泌功能的影响及其作用机制。通过其染毒体外培养的大鼠GC,采用MTT法检测细胞相对活力、ELISA法检测收集培养液中孕酮(P)和雌二醇(E2)的含量、半定量RT-PCR检测激素分泌相关调控基因FSHR、StAR、P450scc和P450arom mRNA的表达、免疫细胞化学染色法检测细胞中特异的卵泡刺激素受体(FSHR)表达,结果显示,T-2毒素可显著降低体外培养的大鼠卵巢颗粒细胞活力,影响其激素的分泌,而转运蛋白StAR则可能是T-2毒素影响颗粒细胞激素分泌的关键位点之一。To study the toxic effects and explore the possible mechanism of T-2 toxin exposure to rat ovarian granulosa cell steroidogenesis in vitro, immature ovarian granulosa cells of wistar rat were primary cultured. After exposure to T-2 toxin 24h, cell viability was detected by MTT assay, content of progester- one （P） and estradiol （E2） in medium were detected by ELISA assay, FSHR, STAR, P450scc and P450arom mRNA expression were detected by semi-quantitative RT-PCR, and expression of follicle stimu- lating hormone receptor-specific receptor（FSHR） in granulosa cell was detected by immunocytochemical staining assay. The results showed that T-2 toxin could significantly decrease the viability of rat ovarian granulosa cells, and affected steroidogenesis, and that StAR maybe one of the key factors to affect the ste- roidogenesis of granulosa cells.

关 键 词：T-2毒素 卵巢颗粒细胞 细胞活力 激素分泌 机制 

分 类 号：S859.79[农业科学—临床兽医学]