AD-MFC中甲醇与硝酸盐的偶合过程与作用机制  

Coupling process and mechanism of methanol oxidation and nitrate reduction in an anodic denitrification microbial fuel cell(AD-MFC)

作　　者：张吉强[1] 郑平[1] 张萌[1] 厉巍[1] 陈慧[1] 蔡琛[1] 谢作甫[1,2] 

机构地区：[1]浙江大学环境工程系,浙江杭州310058 [2]浙江科技学院,浙江杭州310023

出　　处：《化工学报》2013年第9期3404-3411,共8页CIESC Journal

基　　金：国家自然科学基金项目(31070110);高等学校博士学科点专项科研基金(20110101110078);浙江省自然科学基金重点项目(Z5110094);浙江省自然科学基金项目(Y5110266)~~

摘　　要：在双室微生物燃料电池(MFC)阳极内接种反硝化细菌富集培养物,同时加入硝酸盐和甲醇,构建了阳极反硝化微生物燃料电池(AD-MFC),并以批式操作研究了AD-MFC的反硝化产电性能。试验结果表明,在初始硝氮浓度为(100.22±0.62)mg·L-1,COD浓度为(500.40±1.67)mg·L-1的条件下,AD-MFC的最大容积NO3--N和COD去除速率分别达到0.31kg N.m-3.d-1和1.06kg COD.m-3.d-1,最大电压达到(602.80±5.42)mV,相应最大功率密度为(908.42±0.07)mW.m-3。AD-MFC的产电过程是甲醇氧化与硝酸盐还原的偶合过程,电压变化与反硝化作用密切相关,可用于指示反硝化进程。AD-MFC的电压曲线呈现降低-升高-再降低的三阶段特性,其原因是反硝化作用、甲醇降解作用和细胞水解发酵作用依次成为阳极液中的主导反应。An anodic denitrification MFC（AD-MFC）was constructed by inoculating denitrifying bacteria enrichment culture into anode chamber of a dual-chamber MFC,and its capacity of denitrification and electricity production was investigated with methanol as electron donor and nitrate as acceptor in a batch cultivation mode.When initial nitrate and COD concentration were（100.22 ± 0.62）mg·L-1 and（500.40 ± 1.67）mg·L-1,the volumetric nitrogen and COD removal rates reached 0.31 kg N·m-3·d-1 and 1.06 kg COD·m-3·d-1,respectively,coupled with the maximum voltage of（602.80±5.42）mV and the maximum power density of（908.42 ± 0.07）mW·m-3.The results demonstrated that power generation in the AD-MFC was due to a coupling of methanol oxidation and nitrate reduction,and voltage changes were closely related to denitrification,indicating the course of denitrification.Voltage curves of the AD-MFC showed three-phase characteristics of descend phase,ascend phase and redescend phase,which could be ascribed to the succession of denitrification,methanol degradation and cell hydrolysate fermentation as dominant reaction in the anolyte.

关 键 词：微生物燃料电池 反硝化作用 产电性能 脱氮性能 

分 类 号：X703.1[环境科学与工程—环境工程]